Decellularization and cell seeding of whole liver biologic scaffolds composed of
extracellular matrix
#MMPMID25941434
Faulk DM
; Wildemann JD
; Badylak SF
J Clin Exp Hepatol
2015[Mar]; 5
(1
): 69-80
PMID25941434
show ga
The definitive treatment for patients with end-stage liver disease is orthotropic
transplantation. However, this option is limited by the disparity between the
number of patients needing transplantation and the number of available livers.
This issue is becoming more severe as the population ages and as the number of
new cases of end-stage liver failure increases. Patients fortunate enough to
receive a transplant are required to receive immunosuppressive therapy and must
live with the associated morbidity. Whole organ engineering of the liver may
offer a solution to this liver donor shortfall. It has been shown that perfusion
decellularization of a whole allogeneic or xenogeneic liver generates a
three-dimensional ECM scaffold with intact macro and micro architecture of the
native liver. A decellularized liver provides an ideal transplantable scaffold
with all the necessary ultrastructure and signaling cues for cell attachment,
differentiation, vascularization, and function. In this review, an overview of
complementary strategies for creating functional liver grafts suitable for
transplantation is provided. Early milestones have been met by combining stem and
progenitor cells with increasingly complex scaffold materials and culture
conditions.
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