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10.1186/s12864-015-1561-x http://scihub22266oqcxt.onion/10.1186/s12864-015-1561-x C4413528!4413528!25925221     free     free     free Deprecated: Implicit conversion from float 235.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 235.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       BMC+Genomics 2015 ; 16 (1): ä Nephropedia Template TP {{tp|p=25925221|t=2015. Fungal artificial chromosomes for mining of the fungal secondary metabolome |pdf=|usr=}}{{25925221}} gab.com Text Fungal artificial chromosomes for mining of the fungal secondary metabolome JO: BMC Genomics http://www.kidney.de/pget.php?&tw=1&pmid=25925221 #FOAvip Background: With thousands of fungal genomes being sequenced, each genome containing up to 70 secondary metabolite (SM) clusters 30?80 kb in size, breakthrough techniques are needed to characterize this SM wealth. Results: Here we describe a novel system-level methodology for unbiased cloning of intact large SM clusters from a single fungal genome for one-step transformation and expression in a model host. All 56 intact SM clusters from Aspergillus terreus were individually captured in self-replicating fungal artificial chromosomes (FACs) containing both the E. coli F replicon and an Aspergillus autonomously replicating sequence (AMA1). Candidate FACs were successfully shuttled between E. coli and the heterologous expression host A. nidulans. As proof-of-concept, an A. nidulans FAC strain was characterized in a novel liquid chromatography-high resolution mass spectrometry (LC-HRMS) and data analysis pipeline, leading to the discovery of the A. terreus astechrome biosynthetic machinery. Conclusion: The method we present can be used to capture the entire set of intact SM gene clusters and/or pathways from fungal species for heterologous expression in A. nidulans and natural product discovery. Electronic supplementary material: The online version of this article (doi:10.1186/s12864-015-1561-x) contains supplementary material, which is available to authorized users. Twit Text FOAVip Fungal artificial chromosomes for mining of the fungal secondary metabolome ????BMC Genomics http://www.kidney.de/pget.php?&tw=1&pmid=25925221 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=25925221 #FOAvip English Wikipedia <ref>{{Cite pmid|25925221}}</ref> Fungal artificial chromosomes for mining of the fungal secondary metabolome #MMPMID25925221 Bok JW; Ye R; Clevenger KD; Mead D; Wagner M; Krerowicz A; Albright JC; Goering AW; Thomas PM; Kelleher NL; Keller NP; Wu CC BMC Genomics 2015[]; 16 (1): ä PMID25925221show ga Background: With thousands of fungal genomes being sequenced, each genome containing up to 70 secondary metabolite (SM) clusters 30?80 kb in size, breakthrough techniques are needed to characterize this SM wealth. Results: Here we describe a novel system-level methodology for unbiased cloning of intact large SM clusters from a single fungal genome for one-step transformation and expression in a model host. All 56 intact SM clusters from Aspergillus terreus were individually captured in self-replicating fungal artificial chromosomes (FACs) containing both the E. coli F replicon and an Aspergillus autonomously replicating sequence (AMA1). Candidate FACs were successfully shuttled between E. coli and the heterologous expression host A. nidulans. As proof-of-concept, an A. nidulans FAC strain was characterized in a novel liquid chromatography-high resolution mass spectrometry (LC-HRMS) and data analysis pipeline, leading to the discovery of the A. terreus astechrome biosynthetic machinery. Conclusion: The method we present can be used to capture the entire set of intact SM gene clusters and/or pathways from fungal species for heterologous expression in A. nidulans and natural product discovery. Electronic supplementary material: The online version of this article (doi:10.1186/s12864-015-1561-x) contains supplementary material, which is available to authorized users. äFungal artificial chromosomes for mining of the fungal secondary metab(epmc).pdf DeepDyve Pubget Overpricing