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10.1007/978-1-61779-163-5_28 http://scihub22266oqcxt.onion/10.1007/978-1-61779-163-5_28 C4410965!4410965!21761317     free     free     free Warning: file_get_contents(https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pubmed&id=21761317&cmd=llinks): Failed to open stream: HTTP request failed! HTTP/1.1 429 Too Many Requests in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 215 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Methods+Mol+Biol 2011 ; 755 (ä): 327-43 Nephropedia Template TP {{tp|p=21761317|t=2011. Neuronal Type-Specific Gene Expression Profiling and Laser-Capture Microdissection |pdf=|usr=}}{{21761317}} gab.com Text Neuronal Type-Specific Gene Expression Profiling and Laser-Capture Microdissection JO: Methods Mol Biol http://www.kidney.de/pget.php?&tw=1&pmid=21761317 #FOAvip The human brain is an exceptionally heterogeneous structure. In order to gain insight into the neurobiological basis of neural circuit disturbances in various neurologic or psychiatric diseases, it is often important to define the molecular cascades that are associated with these disturbances in a neuronal type-specific manner. This can be achieved by the use of laser microdissection, in combination with molecular techniques such as gene expression profiling. To identify neurons in human postmortem brain tissue, one can use the inherent properties of the neuron, such as pigmentation and morphology or its structural composition through immunohistochemistry (IHC). Here, we describe the isolation of homogeneous neuronal cells and high-quality RNA from human postmortem brain material using a combination of rapid IHC, Nissl staining, or simple morphology with Laser-Capture Microdissection (LCM) or Laser Microdissection (LMD). Twit Text FOAVip Neuronal Type-Specific Gene Expression Profiling and Laser-Capture Microdissection ????Methods Mol Biol http://www.kidney.de/pget.php?&tw=1&pmid=21761317 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=21761317 #FOAvip English Wikipedia <ref>{{Cite pmid|21761317}}</ref> Neuronal Type-Specific Gene Expression Profiling and Laser-Capture Microdissection #MMPMID21761317 Pietersen CY; Lim MP; Macey L; Woo TUW; Sonntag KC Methods Mol Biol 2011[]; 755 (ä): 327-43 PMID21761317show ga The human brain is an exceptionally heterogeneous structure. In order to gain insight into the neurobiological basis of neural circuit disturbances in various neurologic or psychiatric diseases, it is often important to define the molecular cascades that are associated with these disturbances in a neuronal type-specific manner. This can be achieved by the use of laser microdissection, in combination with molecular techniques such as gene expression profiling. To identify neurons in human postmortem brain tissue, one can use the inherent properties of the neuron, such as pigmentation and morphology or its structural composition through immunohistochemistry (IHC). Here, we describe the isolation of homogeneous neuronal cells and high-quality RNA from human postmortem brain material using a combination of rapid IHC, Nissl staining, or simple morphology with Laser-Capture Microdissection (LCM) or Laser Microdissection (LMD). äNeuronal Type-Specific Gene Expression Profiling and Laser-Capture Mic(epmc).pdf DeepDyve Pubget Overpricing