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10.1038/nmeth.3027

http://scihub22266oqcxt.onion/10.1038/nmeth.3027
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C4410700!4410700!25075907
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suck abstract from ncbi


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pmid25075907      Nat+Methods 2014 ; 11 (8): 801-7
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  • Deep mutational scanning: a new style of protein science #MMPMID25075907
  • Fowler DM; Fields S
  • Nat Methods 2014[Aug]; 11 (8): 801-7 PMID25075907show ga
  • Mutagenesis provides insight into proteins, but only recently have assays that couple genotype to phenotype been used to assess the activities of as many as a million mutant versions of a protein in a single experiment. This approach ? ?deep mutational scanning? ? yields large-scale datasets that can reveal intrinsic protein properties, protein behavior within cells and the consequences of human genetic variation. Deep mutational scanning is transforming the study of proteins, but many challenges must be tackled to fulfill its promise.
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