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2015 ; 63
(5
): 340-9
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Characterization of Angiogenesis and Lymphangiogenesis in Human Minor Salivary
Glands with Sjögren s Syndrome
#MMPMID25636309
McCall AD
; Baker OJ
J Histochem Cytochem
2015[May]; 63
(5
): 340-9
PMID25636309
show ga
Angiogenesis has been proposed to play a role in the inflammation observed in
Sjögren's Syndrome (SS). However, no studies have validated the degree of
angiogenesis in salivary glands with SS. Therefore, the goal of this study was to
determine the presence and localization of angiogenesis and lymphangiogenesis in
salivary glands with SS. We used frozen tissue sections from human minor salivary
glands (hMSG) with and without SS in our analyses. To investigate signs of
angiogenesis, hMSG tissue lysates were used to detect levels of the
pro-angiogenic protein vascular endothelial growth factor (VEGF) by western blot
analyses. Additionally, we labeled blood vessels using antibodies specific to
platelet endothelial cell adhesion molecule-1 (PECAM-1) and von Willebrand Factor
(vWF) to determine blood vessel organization and volume fraction using
fluorescence microscopy. Lymphatic vessel organization and volume fraction were
determined using antibodies specific to lymphatic vessel endothelial hyaluronan
receptor (LYVE-1). Our results suggest that expression levels of VEGF are
decreased in hMSG with SS as compared with controls. Interestingly, there were no
significant differences in blood or lymphatic vessel organization or volume
fraction between hMSG with and without SS, suggesting that angiogenesis and
lymphangiogenesis have little impact on the progression of SS.