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10.1016/B978-0-12-374814-0.00009-4 http://scihub22266oqcxt.onion/10.1016/B978-0-12-374814-0.00009-4 C4408312!4408312!21924162     free     free     free Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Methods+Cell+Biol 2011 ; 104 (ä): 151-72 Nephropedia Template TP {{tp|p=21924162|t=2011. Spatiotemporal Control of Embryonic Gene Expression Using Caged Morpholinos |pdf=|usr=}}{{21924162}} gab.com Text Spatiotemporal Control of Embryonic Gene Expression Using Caged Morpholinos JO: Methods Cell Biol http://www.kidney.de/pget.php?&tw=1&pmid=21924162 #FOAvip Embryonic development depends on spatial and temporal control of gene function, and deciphering the molecular mechanisms that underlie pattern formation requires methods for perturbing gene expression with similar precision. Emerging chemical technologies can enable such perturbations, as exemplified by the use of caged morpholino (cMO) oligonucleotides to photo-inactivate genes in zebrafish embryos with spatiotemporal control. This chapter describes general principles for cMO design and methods for cMO assembly in three steps from commercially available reagents. Experimental techniques for the microinjection and photoactivation of these reagents are described in detail, as well as the preparation and application of caged fluorescein dextran (cFD) for labeling irradiated cells. Using these protocols, cMOs can be effective tools for functional genomic studies in zebrafish and other model organisms. Twit Text FOAVip Spatiotemporal Control of Embryonic Gene Expression Using Caged Morpholinos ????Methods Cell Biol http://www.kidney.de/pget.php?&tw=1&pmid=21924162 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=21924162 #FOAvip English Wikipedia <ref>{{Cite pmid|21924162}}</ref> Spatiotemporal Control of Embryonic Gene Expression Using Caged Morpholinos #MMPMID21924162 Shestopalov IA; Chen JK Methods Cell Biol 2011[]; 104 (ä): 151-72 PMID21924162show ga Embryonic development depends on spatial and temporal control of gene function, and deciphering the molecular mechanisms that underlie pattern formation requires methods for perturbing gene expression with similar precision. Emerging chemical technologies can enable such perturbations, as exemplified by the use of caged morpholino (cMO) oligonucleotides to photo-inactivate genes in zebrafish embryos with spatiotemporal control. This chapter describes general principles for cMO design and methods for cMO assembly in three steps from commercially available reagents. Experimental techniques for the microinjection and photoactivation of these reagents are described in detail, as well as the preparation and application of caged fluorescein dextran (cFD) for labeling irradiated cells. Using these protocols, cMOs can be effective tools for functional genomic studies in zebrafish and other model organisms. äSpatiotemporal Control of Embryonic Gene Expression Using Caged Morpho(epmc).pdf DeepDyve Pubget Overpricing