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10.1042/BJ20131668 http://scihub22266oqcxt.onion/10.1042/BJ20131668 C4407826!4407826 !24456382     free     free     free Warning: file_get_contents(https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pubmed&id=24456382 &cmd=llinks): Failed to open stream: HTTP request failed! 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Multiple conformations of a single SNAREpin between two nanodisc membranes reveal diverse pre-fusion states |pdf=|usr=}}{{24456382 }} gab.com Text Multiple conformations of a single SNAREpin between two nanodisc membranes reveal diverse pre-fusion states JO: Biochem J http://www.kidney.de/pget.php?&tw=1&pmid=24456382 #FOAvip SNAREpins must be formed between two membranes to allow vesicle fusion, a required process for neurotransmitter release. Although its post-fusion structure has been well characterized, pre-fusion conformations have been elusive. We used single-molecule FRET and EPR to investigate the SNAREpin assembled between two nanodisc membranes. The SNAREpin shows at least three distinct dynamic states, which might represent pre-fusion intermediates. Although the N-terminal half above the conserved ionic layer maintains a robust helical bundle structure, the membrane-proximal C-terminal half shows high FRET, representing a helical bundle (45%), low FRET, reflecting a frayed conformation (39%) or mid FRET revealing an as-yet unidentified structure (16%). It is generally thought that SNAREpins are trapped at a partially zipped conformation in the pre-fusion state, and complete SNARE (soluble N-ethylmaleimide-sensitive factor-attachment protein receptor) assembly happens concomitantly with membrane fusion. However, our results show that the complete SNARE complex can be formed without membrane fusion, which suggests that the complete SNAREpin formation could precede membrane fusion, providing an ideal access to the fusion regulators such as complexins and synaptotagmin 1. Twit Text FOAVip Multiple conformations of a single SNAREpin between two nanodisc membranes reveal diverse pre-fusion states ????Biochem J http://www.kidney.de/pget.php?&tw=1&pmid=24456382 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=24456382 #FOAvip English Wikipedia <ref>{{Cite pmid|24456382 }}</ref> Multiple conformations of a single SNAREpin between two nanodisc membranes reveal diverse pre-fusion states #MMPMID24456382 Shin J ; Lou X ; Kweon DH ; Shin YK Biochem J 2014[Apr]; 459 (1 ): 95-102 PMID24456382 show ga SNAREpins must be formed between two membranes to allow vesicle fusion, a required process for neurotransmitter release. Although its post-fusion structure has been well characterized, pre-fusion conformations have been elusive. We used single-molecule FRET and EPR to investigate the SNAREpin assembled between two nanodisc membranes. The SNAREpin shows at least three distinct dynamic states, which might represent pre-fusion intermediates. Although the N-terminal half above the conserved ionic layer maintains a robust helical bundle structure, the membrane-proximal C-terminal half shows high FRET, representing a helical bundle (45%), low FRET, reflecting a frayed conformation (39%) or mid FRET revealing an as-yet unidentified structure (16%). It is generally thought that SNAREpins are trapped at a partially zipped conformation in the pre-fusion state, and complete SNARE (soluble N-ethylmaleimide-sensitive factor-attachment protein receptor) assembly happens concomitantly with membrane fusion. However, our results show that the complete SNARE complex can be formed without membrane fusion, which suggests that the complete SNAREpin formation could precede membrane fusion, providing an ideal access to the fusion regulators such as complexins and synaptotagmin 1. |Exocytosis/physiology [MESH] |Membrane Fusion/*physiology [MESH] |Nanoparticles/*chemistry/*metabolism [MESH] |Protein Conformation [MESH]Multiple conformations of a single SNAREpin between two nanodisc membr(epmc).pdf DeepDyve Pubget Overpricing