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10.1007/978-1-62703-739-6_31 http://scihub22266oqcxt.onion/10.1007/978-1-62703-739-6_31 C4407362!4407362 !24623245     free     free     free Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 243.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 243.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Warning: imagejpeg(C:\Inetpub\vhosts\kidney.de\httpdocs\phplern\24623245 .jpg): Failed to open stream: No such file or directory in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 117       Methods+Mol+Biol 2014 ; 1105 (ä): 419-37 Nephropedia Template TP {{tp|p=24623245 |t=2014. Quantitative PCR-based measurement of nuclear and mitochondrial DNA damage and repair in mammalian cells |pdf=|usr=}}{{24623245 }} gab.com Text Quantitative PCR-based measurement of nuclear and mitochondrial DNA damage and repair in mammalian cells JO: Methods Mol Biol http://www.kidney.de/pget.php?&tw=1&pmid=24623245 #FOAvip In this chapter, we describe a gene-specific quantitative PCR (QPCR)-based assay for the measurement of DNA damage, using amplification of long DNA targets. This assay has been used extensively to measure the integrity of both nuclear and mitochondrial genomes exposed to different genotoxins and has proven to be particularly valuable in identifying reactive oxygen species-mediated mitochondrial DNA damage. QPCR can be used to quantify both the formation of DNA damage as well as the kinetics of damage removal. One of the main strengths of the assay is that it permits monitoring the integrity of mtDNA directly from total cellular DNA without the need for isolating mitochondria or a separate step of mitochondrial DNA purification. Here we discuss advantages and limitations of using QPCR to assay DNA damage in mammalian cells. In addition, we give a detailed protocol of the QPCR assay that helps facilitate its successful deployment in any molecular biology laboratory. Twit Text FOAVip Quantitative PCR-based measurement of nuclear and mitochondrial DNA damage and repair in mammalian cells ????Methods Mol Biol http://www.kidney.de/pget.php?&tw=1&pmid=24623245 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=24623245 #FOAvip English Wikipedia <ref>{{Cite pmid|24623245 }}</ref> Quantitative PCR-based measurement of nuclear and mitochondrial DNA damage and repair in mammalian cells #MMPMID24623245 Furda A ; Santos JH ; Meyer JN ; Van Houten B Methods Mol Biol 2014[]; 1105 (ä): 419-37 PMID24623245 show ga In this chapter, we describe a gene-specific quantitative PCR (QPCR)-based assay for the measurement of DNA damage, using amplification of long DNA targets. This assay has been used extensively to measure the integrity of both nuclear and mitochondrial genomes exposed to different genotoxins and has proven to be particularly valuable in identifying reactive oxygen species-mediated mitochondrial DNA damage. QPCR can be used to quantify both the formation of DNA damage as well as the kinetics of damage removal. One of the main strengths of the assay is that it permits monitoring the integrity of mtDNA directly from total cellular DNA without the need for isolating mitochondria or a separate step of mitochondrial DNA purification. Here we discuss advantages and limitations of using QPCR to assay DNA damage in mammalian cells. In addition, we give a detailed protocol of the QPCR assay that helps facilitate its successful deployment in any molecular biology laboratory. |*DNA Damage [MESH] |*DNA Repair [MESH] |*Real-Time Polymerase Chain Reaction [MESH] |Animals [MESH] |Base Sequence [MESH] |Cell Line [MESH] |Cell Nucleus/genetics [MESH] |DNA Copy Number Variations [MESH] |DNA Mutational Analysis [MESH] |DNA Primers/genetics [MESH] |DNA, Mitochondrial/*genetics/isolation & purification [MESH]Quantitative PCR-based measurement of nuclear and mitochondrial DNA da(epmc).pdf DeepDyve Pubget Overpricing