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10.1016/j.devcel.2015.03.002 http://scihub22266oqcxt.onion/10.1016/j.devcel.2015.03.002 C4406947!4406947 !25898166     free     free     free Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 267.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 267.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Warning: imagejpeg(C:\Inetpub\vhosts\kidney.de\httpdocs\phplern\25898166 .jpg): Failed to open stream: No such file or directory in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 117       Dev+Cell 2015 ; 33 (2 ): 163-75 Nephropedia Template TP {{tp|p=25898166 |t=2015. CALM regulates clathrin-coated vesicle size and maturation by directly sensing and driving membrane curvature |pdf=|usr=}}{{25898166 }} gab.com Text CALM regulates clathrin-coated vesicle size and maturation by directly sensing and driving membrane curvature JO: Dev Cell http://www.kidney.de/pget.php?&tw=1&pmid=25898166 #FOAvip The size of endocytic clathrin-coated vesicles (CCVs) is remarkably uniform, suggesting that it is optimized to achieve the appropriate levels of cargo and lipid internalization. The three most abundant proteins in mammalian endocytic CCVs are clathrin and the two cargo-selecting, clathrin adaptors, CALM and AP2. Here we demonstrate that depletion of CALM causes a substantial increase in the ratio of "open" clathrin-coated pits (CCPs) to "necked"/"closed" CCVs and a doubling of CCP/CCV diameter, whereas AP2 depletion has opposite effects. Depletion of either adaptor, however, significantly inhibits endocytosis of transferrin and epidermal growth factor. The phenotypic effects of CALM depletion can be rescued by re-expression of wild-type CALM, but not with CALM that lacks a functional N-terminal, membrane-inserting, curvature-sensing/driving amphipathic helix, the existence and properties of which are demonstrated. CALM is thus a major factor in controlling CCV size and maturation and hence in determining the rates of endocytic cargo uptake. Twit Text FOAVip CALM regulates clathrin-coated vesicle size and maturation by directly sensing and driving membrane curvature ????Dev Cell http://www.kidney.de/pget.php?&tw=1&pmid=25898166 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=25898166 #FOAvip English Wikipedia <ref>{{Cite pmid|25898166 }}</ref> CALM regulates clathrin-coated vesicle size and maturation by directly sensing and driving membrane curvature #MMPMID25898166 Miller SE ; Mathiasen S ; Bright NA ; Pierre F ; Kelly BT ; Kladt N ; Schauss A ; Merrifield CJ ; Stamou D ; Höning S ; Owen DJ Dev Cell 2015[Apr]; 33 (2 ): 163-75 PMID25898166 show ga The size of endocytic clathrin-coated vesicles (CCVs) is remarkably uniform, suggesting that it is optimized to achieve the appropriate levels of cargo and lipid internalization. The three most abundant proteins in mammalian endocytic CCVs are clathrin and the two cargo-selecting, clathrin adaptors, CALM and AP2. Here we demonstrate that depletion of CALM causes a substantial increase in the ratio of "open" clathrin-coated pits (CCPs) to "necked"/"closed" CCVs and a doubling of CCP/CCV diameter, whereas AP2 depletion has opposite effects. Depletion of either adaptor, however, significantly inhibits endocytosis of transferrin and epidermal growth factor. The phenotypic effects of CALM depletion can be rescued by re-expression of wild-type CALM, but not with CALM that lacks a functional N-terminal, membrane-inserting, curvature-sensing/driving amphipathic helix, the existence and properties of which are demonstrated. CALM is thus a major factor in controlling CCV size and maturation and hence in determining the rates of endocytic cargo uptake. |Cell Line, Tumor [MESH] |Cell Membrane/physiology [MESH] |Cell Shape/*genetics [MESH] |Clathrin-Coated Vesicles/*physiology [MESH] |Coated Pits, Cell-Membrane/*physiology [MESH] |Endocytosis [MESH] |Epidermal Growth Factor/metabolism [MESH] |Fatty Acid-Binding Proteins/*genetics [MESH] |HeLa Cells [MESH] |Humans [MESH] |Liposomes/metabolism [MESH] |Monomeric Clathrin Assembly Proteins/*genetics/*physiology [MESH] |Protein Structure, Tertiary [MESH] |R-SNARE Proteins/metabolism [MESH] |RNA Interference [MESH] |RNA, Small Interfering [MESH]CALM regulates clathrin-coated vesicle size and maturation by directly(epmc).pdf DeepDyve Pubget Overpricing