Use my Search Websuite to scan PubMed, PMCentral, Journal Hosts and Journal Archives, FullText. Kick-your-searchterm to multiple Engines kick-your-query now !>

A dictionary by aggregated review articles of nephrology, medicine and the life sciences Your one-stop-run pathway from word to the immediate pdf of peer-reviewed on-topic knowledge.

10.1136/bjo.2010.180869 http://scihub22266oqcxt.onion/10.1136/bjo.2010.180869 C4406415!4406415!21183516     free     free     free Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Br+J+Ophthalmol 2011 ; 95 (5): 727-30 Nephropedia Template TP {{tp|p=21183516|t=2011. Transretinal degeneration in ageing human retina: a multiphoton microscopy analysis |pdf=|usr=}}{{21183516}} gab.com Text Transretinal degeneration in ageing human retina: a multiphoton microscopy analysis JO: Br J Ophthalmol http://www.kidney.de/pget.php?&tw=1&pmid=21183516 #FOAvip Aim: Retinal cell remodelling has been reported as a consistent feature of ageing. However, the degree to which this results in transretinal degeneration is unclear. To address this, the authors used multiphoton microscopy to quantify retinal degeneration in postmortem human eyes of two age groups. Methods: Retinas from six young subjects (18?33 years old) and six older subjects (74?90 years old) were prepared as wholemount preparations. All retinas were stained with 4,6-diamidino-2-phenylindole and imaged by multiphoton confocal microscopy to quantify neuron densities in the retinal ganglion cell layer (RGCL), inner nuclear layer (INL) and outer nuclear layer (ONL). Neurons were counted using automated cell identification algorithms. All retinas were imaged hydrated to minimise tissue artefacts. Results: In both groups, 56% of the area within the central 4 mm eccentricity and 27% of the area with eccentricity between 4 mm and 7 mm were imaged. Compared with young subjects, the peak RGCL neuron loss in the aged subjects (25.5%) was at 1 mm eccentricity. INL and ONL neuron densities significantly decreased at 1?2 mm eccentricity (8.7%) and 0.5?4 mm eccentricity (15.6%) respectively (P <0.05). The reduction in neuron density in the INL corresponded, spatially, to the region with the greatest neuron loss in the RGCL and ONL. Conclusions: This is the first study to correlate neurodegeneration in different populations of cells in the ageing retinas. These data confirm that the greatest neuronal loss occurs in the RGCL and ONL in human ageing retinas, whereas the INL is relatively preserved. Twit Text FOAVip Transretinal degeneration in ageing human retina: a multiphoton microscopy analysis ????Br J Ophthalmol http://www.kidney.de/pget.php?&tw=1&pmid=21183516 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=21183516 #FOAvip English Wikipedia <ref>{{Cite pmid|21183516}}</ref> Transretinal degeneration in ageing human retina: a multiphoton microscopy analysis #MMPMID21183516 Lei Y; Garrahan N; Hermann B; Fautsch MP; Johnson DH; Hernandez MR; Boulton M; Morgan JE Br J Ophthalmol 2011[May]; 95 (5): 727-30 PMID21183516show ga Aim: Retinal cell remodelling has been reported as a consistent feature of ageing. However, the degree to which this results in transretinal degeneration is unclear. To address this, the authors used multiphoton microscopy to quantify retinal degeneration in postmortem human eyes of two age groups. Methods: Retinas from six young subjects (18?33 years old) and six older subjects (74?90 years old) were prepared as wholemount preparations. All retinas were stained with 4,6-diamidino-2-phenylindole and imaged by multiphoton confocal microscopy to quantify neuron densities in the retinal ganglion cell layer (RGCL), inner nuclear layer (INL) and outer nuclear layer (ONL). Neurons were counted using automated cell identification algorithms. All retinas were imaged hydrated to minimise tissue artefacts. Results: In both groups, 56% of the area within the central 4 mm eccentricity and 27% of the area with eccentricity between 4 mm and 7 mm were imaged. Compared with young subjects, the peak RGCL neuron loss in the aged subjects (25.5%) was at 1 mm eccentricity. INL and ONL neuron densities significantly decreased at 1?2 mm eccentricity (8.7%) and 0.5?4 mm eccentricity (15.6%) respectively (P <0.05). The reduction in neuron density in the INL corresponded, spatially, to the region with the greatest neuron loss in the RGCL and ONL. Conclusions: This is the first study to correlate neurodegeneration in different populations of cells in the ageing retinas. These data confirm that the greatest neuronal loss occurs in the RGCL and ONL in human ageing retinas, whereas the INL is relatively preserved. äTransretinal degeneration in ageing human retina: a multiphoton micros(epmc).pdf DeepDyve Pubget Overpricing