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Two distinct populations of doublecortin-positive cells in the perilesional zone
of cortical infarcts
#MMPMID25881110
Kunze A
; Achilles A
; Keiner S
; Witte OW
; Redecker C
BMC Neurosci
2015[Apr]; 16
(?): 20
PMID25881110
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BACKGROUND: Recovery following stroke depends on cellular plasticity in the
perilesional zone (PZ). Doublecortin (DCX), a protein mainly labeling immature
neurons in neurogenic niches is also highly expressed in the vicinity of focal
cortical infarcts. Notably, the number of DCX+ cells positively correlates with
the recovery of functional deficits after stroke though the nature and origin of
these cells remains unclear. RESULTS: In the present study, we aimed to
characterize the population of DCX+ cells in the vicinity of ischemic infarcts in
a mouse model in detail. Employing a photothrombosis model, distinct
immunohistochemical techniques, stereology and confocal microscopy, we show that:
i) DCX+ cells in the perilesional zone do not constitute a homogenous population
and two cell types, stellate and polar cells can be distinguished according to
their morphology. ii) Stellate cells are mainly located in the lateral and medial
vicinity of the insult and express astrocytic markers. iii) Polar cells are found
almost exclusively in the corpus callosum region including in the preserved deep
cortical layers close to the subventricular zone (SVZ). Further, they do not show
any colocalisation of glial markers. Polar morphology and distribution suggest a
migration towards the lesion. CONCLUSIONS: In summary, our findings provide
evidence that in mice DCX+ cells in the perilesional zone of cortical infarcts
comprise a distinct cell population and the majority of cells are of glial
nature.
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