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10.1073/pnas.1422088112 http://scihub22266oqcxt.onion/10.1073/pnas.1422088112 C4403167!4403167 !25825726     free     free     free Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 245.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 245.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Warning: imagejpeg(C:\Inetpub\vhosts\kidney.de\httpdocs\phplern\25825726 .jpg): Failed to open stream: No such file or directory in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 117       Proc+Natl+Acad+Sci+U+S+A 2015 ; 112 (15 ): 4618-23 Nephropedia Template TP {{tp|p=25825726 |t=2015. Characterization of global loss of imprinting in fetal overgrowth syndrome induced by assisted reproduction |pdf=|usr=}}{{25825726 }} gab.com Text Characterization of global loss of imprinting in fetal overgrowth syndrome induced by assisted reproduction JO: Proc Natl Acad Sci U S A http://www.kidney.de/pget.php?&tw=1&pmid=25825726 #FOAvip Embryos generated with the use of assisted reproductive technologies (ART) can develop overgrowth syndromes. In ruminants, the condition is referred to as large offspring syndrome (LOS) and exhibits variable phenotypic abnormalities including overgrowth, enlarged tongue, and abdominal wall defects. These characteristics recapitulate those observed in the human loss-of-imprinting (LOI) overgrowth syndrome Beckwith-Wiedemann (BWS). We have recently shown LOI at the KCNQ1 locus in LOS, the most common epimutation in BWS. Although the first case of ART-induced LOS was reported in 1995, studies have not yet determined the extent of LOI in this condition. Here, we determined allele-specific expression of imprinted genes previously identified in human and/or mouse in day ?105 Bos taurus indicus × Bos taurus taurus F1 hybrid control and LOS fetuses using RNAseq. Our analysis allowed us to determine the monoallelic expression of 20 genes in tissues of control fetuses. LOS fetuses displayed variable LOI compared with controls. Biallelic expression of imprinted genes in LOS was associated with tissue-specific hypomethylation of the normally methylated parental allele. In addition, a positive correlation was observed between body weight and the number of biallelically expressed imprinted genes in LOS fetuses. Furthermore, not only was there loss of allele-specific expression of imprinted genes in LOS, but also differential transcript amounts of these genes between control and overgrown fetuses. In summary, we characterized previously unidentified imprinted genes in bovines and identified misregulation of imprinting at multiple loci in LOS. We concluded that LOS is a multilocus LOI syndrome, as is BWS. Twit Text FOAVip Characterization of global loss of imprinting in fetal overgrowth syndrome induced by assisted reproduction ????Proc Natl Acad Sci U S A http://www.kidney.de/pget.php?&tw=1&pmid=25825726 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=25825726 #FOAvip English Wikipedia <ref>{{Cite pmid|25825726 }}</ref> Characterization of global loss of imprinting in fetal overgrowth syndrome induced by assisted reproduction #MMPMID25825726 Chen Z ; Hagen DE ; Elsik CG ; Ji T ; Morris CJ ; Moon LE ; Rivera RM Proc Natl Acad Sci U S A 2015[Apr]; 112 (15 ): 4618-23 PMID25825726 show ga Embryos generated with the use of assisted reproductive technologies (ART) can develop overgrowth syndromes. In ruminants, the condition is referred to as large offspring syndrome (LOS) and exhibits variable phenotypic abnormalities including overgrowth, enlarged tongue, and abdominal wall defects. These characteristics recapitulate those observed in the human loss-of-imprinting (LOI) overgrowth syndrome Beckwith-Wiedemann (BWS). We have recently shown LOI at the KCNQ1 locus in LOS, the most common epimutation in BWS. Although the first case of ART-induced LOS was reported in 1995, studies have not yet determined the extent of LOI in this condition. Here, we determined allele-specific expression of imprinted genes previously identified in human and/or mouse in day ?105 Bos taurus indicus × Bos taurus taurus F1 hybrid control and LOS fetuses using RNAseq. Our analysis allowed us to determine the monoallelic expression of 20 genes in tissues of control fetuses. LOS fetuses displayed variable LOI compared with controls. Biallelic expression of imprinted genes in LOS was associated with tissue-specific hypomethylation of the normally methylated parental allele. In addition, a positive correlation was observed between body weight and the number of biallelically expressed imprinted genes in LOS fetuses. Furthermore, not only was there loss of allele-specific expression of imprinted genes in LOS, but also differential transcript amounts of these genes between control and overgrown fetuses. In summary, we characterized previously unidentified imprinted genes in bovines and identified misregulation of imprinting at multiple loci in LOS. We concluded that LOS is a multilocus LOI syndrome, as is BWS. |*Genomic Imprinting [MESH] |Alleles [MESH] |Animals [MESH] |Beckwith-Wiedemann Syndrome/embryology/etiology/genetics [MESH] |Cattle/embryology/*genetics [MESH] |DNA Methylation [MESH] |Female [MESH] |Fetus/*abnormalities [MESH] |Gene Expression Profiling [MESH] |Gene Expression Regulation, Developmental [MESH] |Gigantism/embryology/etiology/genetics [MESH] |Humans [MESH] |Male [MESH] |Mice [MESH] |Polymorphism, Single Nucleotide [MESH] |Reproductive Techniques, Assisted/adverse effects/*veterinary [MESH] |Reverse Transcriptase Polymerase Chain Reaction [MESH] |Sequence Analysis, RNA [MESH]Characterization of global loss of imprinting in fetal overgrowth synd(epmc).pdf DeepDyve Pubget Overpricing