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2015 ; 8
(2
): 2226-32
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Effects of Shenqi Neijin powder on activation and apoptosis of hepatic stellate
cells in rats with hepatic fibrosis
#MMPMID25932155
Xie H
; Hou W
; Yang Y
; Yu Y
; Wang F
; Mao J
Int J Clin Exp Med
2015[]; 8
(2
): 2226-32
PMID25932155
show ga
Traditional herbal medicine is usually administrated according to experiences and
practices. We aimed to investigate the anti-fibrotic effects of Shenqi Neijin
powder (SQNJP) in hepatic fibrosis rats induced by carbon tetrachloride (CCl4). A
total of 32 rats were divided into control group, model group, and SQNJP group.
The hydroxyproline content was assayed. Histological features of liver tissues
were determined with different staining methods. Western blotting analysis and
immuno-fluorescence staining were performed to determine the expression of
alpha-smooth muscle actin (?-SMA) and the activation of hepatic stellate cells
(HSCs). Serial sections were stained with ?-SMA immuno-fluorescence staining and
the terminal deoxynucleotidyl transferase-mediated dUTP-biotin nick end labeling
method (TUNEL) in turn to detect the apoptosis of HSCs. Fatty degeneration,
deposition of collagen, and interval of fibers were noticed in rats induced by
CCl4. After administration of SQNJP, remarkable decrease of fatty degeneration,
deposition of collagen, and hydroxyproline content were noticed. Compared with
the model group, significant decrease of ?-SMA protein was noticed after
administration of SQNJP, and remarkable apoptosis of HSCs was noticed after
treating with SQNJP. SQNJP showed anti-fibrotic effects through inhibiting HSCs
activation and inducing apoptosis of HSCs.