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10.1038/nchembio.1793 http://scihub22266oqcxt.onion/10.1038/nchembio.1793 C4402137!4402137!25848930     free     free     free Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Nat+Chem+Biol 2015 ; 11 (5): 316-8 Nephropedia Template TP {{tp|p=25848930|t=2015. Small Molecule-Triggered Cas9 Protein with Improved Genome-Editing Specificity |pdf=|usr=}}{{25848930}} gab.com Text Small Molecule-Triggered Cas9 Protein with Improved Genome-Editing Specificity JO: Nat Chem Biol http://www.kidney.de/pget.php?&tw=1&pmid=25848930 #FOAvip Directly modulating the activity of genome-editing proteins has the potential to increase their specificity by reducing activity following target locus modification. We developed Cas9 nucleases that are activated by the presence of a cell-permeable small molecule by inserting an evolved 4-hydroxytamoxifen (4-HT)-responsive intein at specific positions in Cas9. In human cells, conditionally active Cas9s modify target genomic sites with up to 25-fold higher specificity than wild-type Cas9. Twit Text FOAVip Small Molecule-Triggered Cas9 Protein with Improved Genome-Editing Specificity ????Nat Chem Biol http://www.kidney.de/pget.php?&tw=1&pmid=25848930 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=25848930 #FOAvip English Wikipedia <ref>{{Cite pmid|25848930}}</ref> Small Molecule-Triggered Cas9 Protein with Improved Genome-Editing Specificity #MMPMID25848930 Davis KM; Pattanayak V; Thompson DB; Zuris JA; Liu DR Nat Chem Biol 2015[May]; 11 (5): 316-8 PMID25848930show ga Directly modulating the activity of genome-editing proteins has the potential to increase their specificity by reducing activity following target locus modification. We developed Cas9 nucleases that are activated by the presence of a cell-permeable small molecule by inserting an evolved 4-hydroxytamoxifen (4-HT)-responsive intein at specific positions in Cas9. In human cells, conditionally active Cas9s modify target genomic sites with up to 25-fold higher specificity than wild-type Cas9. äSmall Molecule-Triggered Cas9 Protein with Improved Genome-Editing Spe(epmc).pdf DeepDyve Pubget Overpricing