Warning: file_get_contents(https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pubmed&id=25832005
&cmd=llinks): Failed to open stream: HTTP request failed! HTTP/1.1 429 Too Many Requests
in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 215
Stem cell conditioned culture media attenuated albumin-induced
epithelial-mesenchymal transition in renal tubular cells
#MMPMID25832005
Hu J
; Zhu Q
; Li PL
; Wang W
; Yi F
; Li N
Cell Physiol Biochem
2015[]; 35
(5
): 1719-28
PMID25832005
show ga
BACKGROUND: Proteinuria-induced epithelial-mesenchymal transition (EMT) plays an
important role in progressive renal tubulointerstitial fibrosis in chronic renal
disease. Stem cell therapy has been used for different diseases. Stem cell
conditioned culture media (SCM) exhibits similar beneficial effects as stem cell
therapy. The present study tested the hypothesis that SCM inhibits
albumin-induced EMT in cultured renal tubular cells. METHODS: Rat renal tubular
cells were treated with/without albumin (20 µmg/ml) plus SCM or control cell
media (CCM). EMT markers and inflammatory factors were measured by Western blot
and fluorescent images. RESULTS: Albumin induced EMT as shown by significant
decreases in levels of epithelial marker E-cadherin, increases in mesenchymal
markers fibroblast-specific protein 1 and ?-smooth muscle actin, and elevations
in collagen I. SCM inhibited all these changes. Meanwhile, albumin induced NF-?B
translocation from cytosol into nucleus and that SCM blocked the nuclear
translocation of NF-?B. Albumin also increased the levels of pro-inflammatory
factor monocyte chemoattractant protein-1 (MCP)-1 by nearly 30 fold compared with
control. SCM almost abolished albumin-induced increase of MCP-1. CONCLUSION:
These results suggest that SCM attenuated albumin-induced EMT in renal tubular
cells via inhibiting activation of inflammatory factors, which may serve as a new
therapeutic approach for chronic kidney diseases.
free
free
free
