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.jpg): Failed to open stream: No such file or directory in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 117 J+Cell+Mol+Med
2007 ; 11
(5
): 1175-84
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Myocardial interstitial Cajal-like cells (ICLC) and their nanostructural
relationships with intercalated discs: shed vesicles as intermediates
#MMPMID17979892
Mandache E
; Popescu LM
; Gherghiceanu M
J Cell Mol Med
2007[Sep]; 11
(5
): 1175-84
PMID17979892
show ga
Intercalated discs (ID) are complex junctional units that connect cardiac
myocytes mechanically and electrochemically. However, there is limited
information concerning the cardiomyocyte interaction with interstitial non-muscle
cells. Our previous studies showed that myocardial interstitial Cajal-like cells
(ICLC) are located in between cardiomyocytes, blood capillaries and nerve fibres.
Typically, ICLC have several very long, moniliform, cytoplasmic processes which
establish closed contacts with nerve fibres, as well as each other. We report
here ultrastructural evidence concerning the relationships of ICLC processes with
ID. The ICLC cytoplasmic prolongations (tens micrometers length) preferentially
pass by or stop nearby the ID. Transmission electron microscopy emphasized three
distinct connecting features between the tips of ICLC extensions and myocytes at
the 'mouth' of ID: free or budding shed vesicles, exocytotic multi-vesicular
bodies and direct contacts. In the last case, electron-dense repetitive
nanostructures ('pillars') (35-40 nm high and 100-150 nm wide, similar to
adhesion molecules) fasten the ICLC to the myocytes. All these features suggest a
juxtacrine and/or paracrine intercellular mutual modulation of ICLC and
cardiomyocytes in the microenvironment of ID, possibly monitoring the cardiac
functions, particularly the electrical activity.