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10.1111/febs.13234 http://scihub22266oqcxt.onion/10.1111/febs.13234 C4400230!4400230 !25688651     free     free     free Warning: file_get_contents(https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pubmed&id=25688651 &cmd=llinks): Failed to open stream: HTTP request failed! 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Simultaneous in vivo imaging of blood and lymphatic vessel growth in Prox1-GFP/Flk1::myr-mCherry mice |pdf=|usr=}}{{25688651 }} gab.com Text Simultaneous in vivo imaging of blood and lymphatic vessel growth in Prox1-GFP/Flk1::myr-mCherry mice JO: FEBS J http://www.kidney.de/pget.php?&tw=1&pmid=25688651 #FOAvip The ability to visually observe angiogenesis and lymphangiogenesis simultaneously and repeatedly in living animals would greatly enhance our understanding of the inter-dependence of these processes. To generate a mouse model that allows such visualization via in vivo fluorescence imaging, we crossed Prox1-GFP mice with Flk1::myr-mCherry mice to generate Prox1-GFP/Flk1::myr-mCherry mice, in which lymphatic vessels emit green fluorescence and blood vessels emit red fluorescence. Corneal neovascularization was induced in these mice using three injury models: implantation of a vascular endothelial growth factor (VEGF) pellet, implantation of a basic fibroblast growth factor (bFGF) pellet, and alkali burn injury. Vessel growth was observed in vivo by stereomicroscopy on days 0, 3, 7 and 10 after pellet implantation or alkali injury as well as in flat-mounted corneas via confocal microscopy after the final in vivo imaging time point. We observed blood and lymphatic vessel growth in all three models, with the most significant growth occurring from days 0-7. Upon VEGF stimulation, the growth kinetics of blood and lymphatic vessels were similar. Blood vessels exhibited similar growth patterns in VEGF- and bFGF-stimulated corneas. Alkali burn injury induced robust angiogenesis and lymphangiogenesis. The intrinsic fluorescence of blood and lymphatic endothelial cells in Prox1-GFP/Flk1::myr-mCherry mice permitted simultaneous in vivo imaging of angiogenesis and lymphangiogenesis. This allowed us to differentiate the processes as well as observe their inter-dependence, and will be valuable in development of therapies targeting angiogenesis and/or lymphangiogenesis. Twit Text FOAVip Simultaneous in vivo imaging of blood and lymphatic vessel growth in Prox1-GFP/Flk1::myr-mCherry mice ????FEBS J http://www.kidney.de/pget.php?&tw=1&pmid=25688651 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=25688651 #FOAvip English Wikipedia <ref>{{Cite pmid|25688651 }}</ref> Simultaneous in vivo imaging of blood and lymphatic vessel growth in Prox1-GFP/Flk1::myr-mCherry mice #MMPMID25688651 Zhu J ; Dugas-Ford J ; Chang M ; Purta P ; Han KY ; Hong YK ; Dickinson ME ; Rosenblatt MI ; Chang JH ; Azar DT FEBS J 2015[Apr]; 282 (8 ): 1458-1467 PMID25688651 show ga The ability to visually observe angiogenesis and lymphangiogenesis simultaneously and repeatedly in living animals would greatly enhance our understanding of the inter-dependence of these processes. To generate a mouse model that allows such visualization via in vivo fluorescence imaging, we crossed Prox1-GFP mice with Flk1::myr-mCherry mice to generate Prox1-GFP/Flk1::myr-mCherry mice, in which lymphatic vessels emit green fluorescence and blood vessels emit red fluorescence. Corneal neovascularization was induced in these mice using three injury models: implantation of a vascular endothelial growth factor (VEGF) pellet, implantation of a basic fibroblast growth factor (bFGF) pellet, and alkali burn injury. Vessel growth was observed in vivo by stereomicroscopy on days 0, 3, 7 and 10 after pellet implantation or alkali injury as well as in flat-mounted corneas via confocal microscopy after the final in vivo imaging time point. We observed blood and lymphatic vessel growth in all three models, with the most significant growth occurring from days 0-7. Upon VEGF stimulation, the growth kinetics of blood and lymphatic vessels were similar. Blood vessels exhibited similar growth patterns in VEGF- and bFGF-stimulated corneas. Alkali burn injury induced robust angiogenesis and lymphangiogenesis. The intrinsic fluorescence of blood and lymphatic endothelial cells in Prox1-GFP/Flk1::myr-mCherry mice permitted simultaneous in vivo imaging of angiogenesis and lymphangiogenesis. This allowed us to differentiate the processes as well as observe their inter-dependence, and will be valuable in development of therapies targeting angiogenesis and/or lymphangiogenesis. |*Corneal Neovascularization [MESH] |*Lymphangiogenesis [MESH] |Alkalies/toxicity [MESH] |Animals [MESH] |Burns, Chemical/metabolism/pathology [MESH] |Corneal Injuries/metabolism/*pathology [MESH] |Female [MESH] |Fibroblast Growth Factor 2/metabolism [MESH] |Green Fluorescent Proteins/*metabolism [MESH] |Homeodomain Proteins/*physiology [MESH] |Image Processing, Computer-Assisted [MESH] |Immunoenzyme Techniques [MESH] |Luminescent Proteins/*metabolism [MESH] |Lymphatic Vessels/metabolism/pathology [MESH] |Male [MESH] |Mice [MESH] |Mice, Inbred C57BL [MESH] |Microscopy, Confocal [MESH] |Prospero-Related Homeobox 1 Protein [MESH] |Red Fluorescent Protein [MESH] |Tumor Suppressor Proteins/*physiology [MESH] |Vascular Endothelial Growth Factor A/metabolism [MESH]Simultaneous in vivo imaging of blood and lymphatic vessel growth in (epmc).pdf DeepDyve Pubget Overpricing