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10.1016/j.ymben.2014.09.007 http://scihub22266oqcxt.onion/10.1016/j.ymben.2014.09.007 C4399830!4399830!25448400     free     free     free Deprecated: Implicit conversion from float 215.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Metab+Eng 2015 ; 27 (ä): 115-35 Nephropedia Template TP {{tp|p=25448400|t=2015. Review of methods to probe single cell metabolism and bioenergetics |pdf=|usr=}}{{25448400}} gab.com Text Review of methods to probe single cell metabolism and bioenergetics JO: Metab Eng http://www.kidney.de/pget.php?&tw=1&pmid=25448400 #FOAvip Single cell investigations have enabled unexpected discoveries, such as the existence of biological noise and phenotypic switching in infection, metabolism and treatment. Herein, we review methods that enable such single cell investigations specific to metabolism and bioenergetics. Firstly, we discuss how to isolate and immobilize individuals from a cell suspension, including both permanent and reversible approaches. We also highlight specific advances in microbiology for its implications in metabolic engineering. Methods for probing single cell physiology and metabolism are subsequently reviewed. The primary focus therein is on dynamic and high-content profiling strategies based on label-free and fluorescence microspectroscopy and microscopy. Non-dynamic approaches, such as mass spectrometry and nuclear magnetic resonance, are also briefly discussed. Twit Text FOAVip Review of methods to probe single cell metabolism and bioenergetics ????Metab Eng http://www.kidney.de/pget.php?&tw=1&pmid=25448400 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=25448400 #FOAvip English Wikipedia <ref>{{Cite pmid|25448400}}</ref> Review of methods to probe single cell metabolism and bioenergetics #MMPMID25448400 Vasdekis AE; Stephanopoulos G Metab Eng 2015[Jan]; 27 (ä): 115-35 PMID25448400show ga Single cell investigations have enabled unexpected discoveries, such as the existence of biological noise and phenotypic switching in infection, metabolism and treatment. Herein, we review methods that enable such single cell investigations specific to metabolism and bioenergetics. Firstly, we discuss how to isolate and immobilize individuals from a cell suspension, including both permanent and reversible approaches. We also highlight specific advances in microbiology for its implications in metabolic engineering. Methods for probing single cell physiology and metabolism are subsequently reviewed. The primary focus therein is on dynamic and high-content profiling strategies based on label-free and fluorescence microspectroscopy and microscopy. Non-dynamic approaches, such as mass spectrometry and nuclear magnetic resonance, are also briefly discussed. äReview of methods to probe single cell metabolism and bioenergetics (epmc).pdf DeepDyve Pubget Overpricing