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10.1016/B978-0-12-801185-0.00012-X http://scihub22266oqcxt.onion/10.1016/B978-0-12-801185-0.00012-X C4398311!4398311!25398344     free     free     free Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Methods+Enzymol 2014 ; 546 (ä): 251-71 Nephropedia Template TP {{tp|p=25398344|t=2014. Creating cancer translocations in human cells using Cas9 DSBs and nCas9 paired nicks |pdf=|usr=}}{{25398344}} gab.com Text Creating cancer translocations in human cells using Cas9 DSBs and nCas9 paired nicks JO: Methods Enzymol http://www.kidney.de/pget.php?&tw=1&pmid=25398344 #FOAvip Recurrent chromosomal translocations are found in numerous of tumor types, often leading to the formation and expression of fusion genes with oncogenic potential. Creating chromosomal translocations at the relevant endogenous loci, rather than just ectopically expressing the fusion genes, opens new possibilities for better characterizing molecular mechanisms driving tumor formation. In this chapter, we describe methods to create cancer translocations in human cells. DSBs or paired nicks generated by either wild-type Cas9 or the Cas9 nickase, respectively, are used to induce translocations at the relevant loci. Using different PCR-based methods, we also explain how to quantify translocation frequency and to analyze breakpoint junctions in the cells of interest. In addition, PCR detection of translocations is used as a very sensitive method to detect off-target effects, which has general utility. Twit Text FOAVip Creating cancer translocations in human cells using Cas9 DSBs and nCas9 paired nicks ????Methods Enzymol http://www.kidney.de/pget.php?&tw=1&pmid=25398344 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=25398344 #FOAvip English Wikipedia <ref>{{Cite pmid|25398344}}</ref> Creating cancer translocations in human cells using Cas9 DSBs and nCas9 paired nicks #MMPMID25398344 Renouf B; Piganeau M; Ghezraoui H; Jasin M; Brunet E Methods Enzymol 2014[]; 546 (ä): 251-71 PMID25398344show ga Recurrent chromosomal translocations are found in numerous of tumor types, often leading to the formation and expression of fusion genes with oncogenic potential. Creating chromosomal translocations at the relevant endogenous loci, rather than just ectopically expressing the fusion genes, opens new possibilities for better characterizing molecular mechanisms driving tumor formation. In this chapter, we describe methods to create cancer translocations in human cells. DSBs or paired nicks generated by either wild-type Cas9 or the Cas9 nickase, respectively, are used to induce translocations at the relevant loci. Using different PCR-based methods, we also explain how to quantify translocation frequency and to analyze breakpoint junctions in the cells of interest. In addition, PCR detection of translocations is used as a very sensitive method to detect off-target effects, which has general utility. äCreating cancer translocations in human cells using Cas9 DSBs and nCas(epmc).pdf DeepDyve Pubget Overpricing