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10.1126/science.1254445 http://scihub22266oqcxt.onion/10.1126/science.1254445 C4398027!4398027!25123483     free     free     free Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Science 2014 ; 345 (6201): 1184-8 Nephropedia Template TP {{tp|p=25123483|t=2014. Prevention of muscular dystrophy in mice by CRISPR/Cas9?mediated editing of germline DNA |pdf=|usr=}}{{25123483}} gab.com Text Prevention of muscular dystrophy in mice by CRISPR/Cas9 mediated editing of germline DNA JO: Science http://www.kidney.de/pget.php?&tw=1&pmid=25123483 #FOAvip Duchenne muscular dystrophy (DMD) is an inherited X-linked disease caused by mutations in the gene encoding dystrophin, a protein required for muscle fiber integrity. DMD is characterized by progressive muscle weakness and a shortened life span, and there is no effective treatment. We used clustered regularly interspaced short palindromic repeat/Cas9 (CRISPR/Cas9)?mediated genome editing to correct the dystrophin gene (Dmd) mutation in the germ line of mdx mice, a model for DMD, and then monitored muscle structure and function. Genome editing produced genetically mosaic animals containing 2 to 100% correction of the Dmd gene. The degree of muscle phenotypic rescue in mosaic mice exceeded the efficiency of gene correction, likely reflecting an advantage of the corrected cells and their contribution to regenerating muscle. With the anticipated technological advances that will facilitate genome editing of postnatal somatic cells, this strategy may one day allow correction of disease-causing mutations in the muscle tissue of patients with DMD. Twit Text FOAVip Prevention of muscular dystrophy in mice by CRISPR/Cas9 mediated editing of germline DNA ????Science http://www.kidney.de/pget.php?&tw=1&pmid=25123483 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=25123483 #FOAvip English Wikipedia <ref>{{Cite pmid|25123483}}</ref> Prevention of muscular dystrophy in mice by CRISPR/Cas9?mediated editing of germline DNA #MMPMID25123483 Long C; McAnally JR; Shelton JM; Mireault AA; Bassel-Duby R; Olson EN Science 2014[Sep]; 345 (6201): 1184-8 PMID25123483show ga Duchenne muscular dystrophy (DMD) is an inherited X-linked disease caused by mutations in the gene encoding dystrophin, a protein required for muscle fiber integrity. DMD is characterized by progressive muscle weakness and a shortened life span, and there is no effective treatment. We used clustered regularly interspaced short palindromic repeat/Cas9 (CRISPR/Cas9)?mediated genome editing to correct the dystrophin gene (Dmd) mutation in the germ line of mdx mice, a model for DMD, and then monitored muscle structure and function. Genome editing produced genetically mosaic animals containing 2 to 100% correction of the Dmd gene. The degree of muscle phenotypic rescue in mosaic mice exceeded the efficiency of gene correction, likely reflecting an advantage of the corrected cells and their contribution to regenerating muscle. With the anticipated technological advances that will facilitate genome editing of postnatal somatic cells, this strategy may one day allow correction of disease-causing mutations in the muscle tissue of patients with DMD. äPrevention of muscular dystrophy in mice by CRISPR/Cas9?mediated editi(epmc).pdf DeepDyve Pubget Overpricing