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10.1016/j.jconrel.2015.01.004 http://scihub22266oqcxt.onion/10.1016/j.jconrel.2015.01.004 C4395535!4395535 !25575866     free     free     free Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 243.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 243.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 243.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Warning: imagejpeg(C:\Inetpub\vhosts\kidney.de\httpdocs\phplern\25575866 .jpg): Failed to open stream: No such file or directory in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 117       J+Control+Release 2015 ; 205 (ä): 128-33 Nephropedia Template TP {{tp|p=25575866 |t=2015. Development of porous PLGA/PEI1 8k biodegradable microspheres for the delivery of mesenchymal stem cells (MSCs) |pdf=|usr=}}{{25575866 }} gab.com Text Development of porous PLGA/PEI1 8k biodegradable microspheres for the delivery of mesenchymal stem cells (MSCs) JO: J Control Release http://www.kidney.de/pget.php?&tw=1&pmid=25575866 #FOAvip Multipotent mesenchymal stem cells (MSCs) promise a therapeutic alternative for many debilitating and incurable diseases. However, one of the major limitations for the therapeutic application of human MSC (hMSC) is the lengthy ex vivo expansion time for preparing a sufficient amount of cells due to the low engraftment rate after transplantation. To solve this conundrum, a porous biodegradable polymeric microsphere was investigated as a potential scaffold for the delivery of MSCs. The modified water/oil/water (W1/O/W2) double emulsion solvent evaporation method was used for the construction of porous microspheres. PEI1.8k was blended with poly(lactic-co-glycolic acid) (PLGA) to enhance electrostatic cellular attachment to the microspheres. The porous PLGA/PEI1.8k (PPP) particles demonstrated an average particle size of 290?m and an average pore size of 14.3?m, providing a micro-carrier for the MSC delivery. PPP particles allowed for better attachment of rMSCs than non-porous PLGA/PEI1.8k (NPP) particles and non-porous (NP) and porous PLGA (PP) microspheres. rMSC successfully grew on the PPP particles for 2weeks in vitro. Next, PPP particles loaded with 3 different amounts of hMSC showed increased in vivo engraftment rates and maintained the stemness characteristics of hMSC compared with hMSCs-alone group in rats 2weeks after intramyocardial administration. These customized PPP particles for MSC delivery are a biodegradable and injectable scaffold that can be used for clinical applications. Twit Text FOAVip Development of porous PLGA/PEI1 8k biodegradable microspheres for the delivery of mesenchymal stem cells (MSCs) ????J Control Release http://www.kidney.de/pget.php?&tw=1&pmid=25575866 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=25575866 #FOAvip English Wikipedia <ref>{{Cite pmid|25575866 }}</ref> Development of porous PLGA/PEI1 8k biodegradable microspheres for the delivery of mesenchymal stem cells (MSCs) #MMPMID25575866 Lee YS ; Lim KS ; Oh JE ; Yoon AR ; Joo WS ; Kim HS ; Yun CO ; Kim SW J Control Release 2015[May]; 205 (ä): 128-33 PMID25575866 show ga Multipotent mesenchymal stem cells (MSCs) promise a therapeutic alternative for many debilitating and incurable diseases. However, one of the major limitations for the therapeutic application of human MSC (hMSC) is the lengthy ex vivo expansion time for preparing a sufficient amount of cells due to the low engraftment rate after transplantation. To solve this conundrum, a porous biodegradable polymeric microsphere was investigated as a potential scaffold for the delivery of MSCs. The modified water/oil/water (W1/O/W2) double emulsion solvent evaporation method was used for the construction of porous microspheres. PEI1.8k was blended with poly(lactic-co-glycolic acid) (PLGA) to enhance electrostatic cellular attachment to the microspheres. The porous PLGA/PEI1.8k (PPP) particles demonstrated an average particle size of 290?m and an average pore size of 14.3?m, providing a micro-carrier for the MSC delivery. PPP particles allowed for better attachment of rMSCs than non-porous PLGA/PEI1.8k (NPP) particles and non-porous (NP) and porous PLGA (PP) microspheres. rMSC successfully grew on the PPP particles for 2weeks in vitro. Next, PPP particles loaded with 3 different amounts of hMSC showed increased in vivo engraftment rates and maintained the stemness characteristics of hMSC compared with hMSCs-alone group in rats 2weeks after intramyocardial administration. These customized PPP particles for MSC delivery are a biodegradable and injectable scaffold that can be used for clinical applications. |*Regeneration [MESH] |*Tissue Scaffolds [MESH] |Animals [MESH] |Cell Adhesion [MESH] |Cell Differentiation [MESH] |Cell Lineage [MESH] |Cell Proliferation [MESH] |Cell Survival [MESH] |Cells, Cultured [MESH] |Disease Models, Animal [MESH] |Graft Survival [MESH] |Humans [MESH] |Imines/*chemistry [MESH] |Injections, Intralesional [MESH] |Lactic Acid/*chemistry [MESH] |Male [MESH] |Mesenchymal Stem Cell Transplantation/*methods [MESH] |Mesenchymal Stem Cells/*physiology [MESH] |Microspheres [MESH] |Myocardial Infarction/pathology/*surgery [MESH] |Myocardium/*pathology [MESH] |Particle Size [MESH] |Polyethylenes/*chemistry [MESH] |Polyglycolic Acid/*chemistry [MESH] |Polylactic Acid-Polyglycolic Acid Copolymer [MESH] |Porosity [MESH] |Rats, Sprague-Dawley [MESH]Development of porous PLGA/PEI1 8k biodegradable microspheres for the (epmc).pdf DeepDyve Pubget Overpricing