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10.1083/jcb.201501081 http://scihub22266oqcxt.onion/10.1083/jcb.201501081 C4395480!4395480 !25847537     free     free     free Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Warning: imagejpeg(C:\Inetpub\vhosts\kidney.de\httpdocs\phplern\25847537 .jpg): Failed to open stream: No such file or directory in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 117       J+Cell+Biol 2015 ; 209 (1 ): 163-80 Nephropedia Template TP {{tp|p=25847537 |t=2015. Open source software for quantification of cell migration, protrusions, and fluorescence intensities |pdf=|usr=}}{{25847537 }} gab.com Text Open source software for quantification of cell migration, protrusions, and fluorescence intensities JO: J Cell Biol http://www.kidney.de/pget.php?&tw=1&pmid=25847537 #FOAvip Cell migration is frequently accompanied by changes in cell morphology (morphodynamics) on a range of spatial and temporal scales. Despite recent advances in imaging techniques, the application of unbiased computational image analysis methods for morphodynamic quantification is rare. For example, manual analysis using kymographs is still commonplace, often caused by lack of access to user-friendly, automated tools. We now describe software designed for the automated quantification of cell migration and morphodynamics. Implemented as a plug-in for the open-source platform, ImageJ, ADAPT is capable of rapid, automated analysis of migration and membrane protrusions, together with associated fluorescently labeled proteins, across multiple cells. We demonstrate the ability of the software by quantifying variations in cell population migration rates on different extracellular matrices. We also show that ADAPT can detect and morphologically profile filopodia. Finally, we have used ADAPT to compile an unbiased description of a "typical" bleb formed at the plasma membrane and quantify the effect of Arp2/3 complex inhibition on bleb retraction. Twit Text FOAVip Open source software for quantification of cell migration, protrusions, and fluorescence intensities ????J Cell Biol http://www.kidney.de/pget.php?&tw=1&pmid=25847537 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=25847537 #FOAvip English Wikipedia <ref>{{Cite pmid|25847537 }}</ref> Open source software for quantification of cell migration, protrusions, and fluorescence intensities #MMPMID25847537 Barry DJ ; Durkin CH ; Abella JV ; Way M J Cell Biol 2015[Apr]; 209 (1 ): 163-80 PMID25847537 show ga Cell migration is frequently accompanied by changes in cell morphology (morphodynamics) on a range of spatial and temporal scales. Despite recent advances in imaging techniques, the application of unbiased computational image analysis methods for morphodynamic quantification is rare. For example, manual analysis using kymographs is still commonplace, often caused by lack of access to user-friendly, automated tools. We now describe software designed for the automated quantification of cell migration and morphodynamics. Implemented as a plug-in for the open-source platform, ImageJ, ADAPT is capable of rapid, automated analysis of migration and membrane protrusions, together with associated fluorescently labeled proteins, across multiple cells. We demonstrate the ability of the software by quantifying variations in cell population migration rates on different extracellular matrices. We also show that ADAPT can detect and morphologically profile filopodia. Finally, we have used ADAPT to compile an unbiased description of a "typical" bleb formed at the plasma membrane and quantify the effect of Arp2/3 complex inhibition on bleb retraction. |*Cell Movement [MESH] |*Software [MESH] |Actin-Related Protein 2-3 Complex/metabolism [MESH] |Apoptosis [MESH] |Cell Shape [MESH] |Cell Tracking [MESH] |HeLa Cells [MESH] |Humans [MESH] |Image Processing, Computer-Assisted [MESH] |Microscopy, Fluorescence [MESH] |Pseudopodia/ultrastructure [MESH]Open source software for quantification of cell migration, protrusions(epmc).pdf DeepDyve Pubget Overpricing