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10.1073/pnas.1423819112 http://scihub22266oqcxt.onion/10.1073/pnas.1423819112 C4394320!4394320 !25831520     free     free     free Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 245.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 245.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Warning: imagejpeg(C:\Inetpub\vhosts\kidney.de\httpdocs\phplern\25831520 .jpg): Failed to open stream: No such file or directory in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 117       Proc+Natl+Acad+Sci+U+S+A 2015 ; 112 (14 ): 4304-9 Nephropedia Template TP {{tp|p=25831520 |t=2015. SWI/SNF chromatin-remodeling complexes function in noncoding RNA-dependent assembly of nuclear bodies |pdf=|usr=}}{{25831520 }} gab.com Text SWI/SNF chromatin-remodeling complexes function in noncoding RNA-dependent assembly of nuclear bodies JO: Proc Natl Acad Sci U S A http://www.kidney.de/pget.php?&tw=1&pmid=25831520 #FOAvip Paraspeckles are subnuclear structures that form around nuclear paraspeckle assembly transcript 1 (NEAT1) long noncoding RNA (lncRNA). Recently, paraspeckles were shown to be functional nuclear bodies involved in stress responses and the development of specific organs. Paraspeckle formation is initiated by transcription of the NEAT1 chromosomal locus and proceeds in conjunction with NEAT1 lncRNA biogenesis and a subsequent assembly step involving >40 paraspeckle proteins (PSPs). In this study, subunits of SWItch/Sucrose NonFermentable (SWI/SNF) chromatin-remodeling complexes were identified as paraspeckle components that interact with PSPs and NEAT1 lncRNA. EM observations revealed that SWI/SNF complexes were enriched in paraspeckle subdomains depleted of chromatin. Knockdown of SWI/SNF components resulted in paraspeckle disintegration, but mutation of the ATPase domain of the catalytic subunit BRG1 did not affect paraspeckle integrity, indicating that the essential role of SWI/SNF complexes in paraspeckle formation does not require their canonical activity. Knockdown of SWI/SNF complexes barely affected the levels of known essential paraspeckle components, but markedly diminished the interactions between essential PSPs, suggesting that SWI/SNF complexes facilitate organization of the PSP interaction network required for intact paraspeckle assembly. The interactions between SWI/SNF components and essential PSPs were maintained in NEAT1-depleted cells, suggesting that SWI/SNF complexes not only facilitate interactions between PSPs, but also recruit PSPs during paraspeckle assembly. SWI/SNF complexes were also required for Satellite III lncRNA-dependent formation of nuclear stress bodies under heat-shock conditions. Our data suggest the existence of a common mechanism underlying the formation of lncRNA-dependent nuclear body architectures in mammalian cells. Twit Text FOAVip SWI/SNF chromatin-remodeling complexes function in noncoding RNA-dependent assembly of nuclear bodies ????Proc Natl Acad Sci U S A http://www.kidney.de/pget.php?&tw=1&pmid=25831520 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=25831520 #FOAvip English Wikipedia <ref>{{Cite pmid|25831520 }}</ref> SWI/SNF chromatin-remodeling complexes function in noncoding RNA-dependent assembly of nuclear bodies #MMPMID25831520 Kawaguchi T ; Tanigawa A ; Naganuma T ; Ohkawa Y ; Souquere S ; Pierron G ; Hirose T Proc Natl Acad Sci U S A 2015[Apr]; 112 (14 ): 4304-9 PMID25831520 show ga Paraspeckles are subnuclear structures that form around nuclear paraspeckle assembly transcript 1 (NEAT1) long noncoding RNA (lncRNA). Recently, paraspeckles were shown to be functional nuclear bodies involved in stress responses and the development of specific organs. Paraspeckle formation is initiated by transcription of the NEAT1 chromosomal locus and proceeds in conjunction with NEAT1 lncRNA biogenesis and a subsequent assembly step involving >40 paraspeckle proteins (PSPs). In this study, subunits of SWItch/Sucrose NonFermentable (SWI/SNF) chromatin-remodeling complexes were identified as paraspeckle components that interact with PSPs and NEAT1 lncRNA. EM observations revealed that SWI/SNF complexes were enriched in paraspeckle subdomains depleted of chromatin. Knockdown of SWI/SNF components resulted in paraspeckle disintegration, but mutation of the ATPase domain of the catalytic subunit BRG1 did not affect paraspeckle integrity, indicating that the essential role of SWI/SNF complexes in paraspeckle formation does not require their canonical activity. Knockdown of SWI/SNF complexes barely affected the levels of known essential paraspeckle components, but markedly diminished the interactions between essential PSPs, suggesting that SWI/SNF complexes facilitate organization of the PSP interaction network required for intact paraspeckle assembly. The interactions between SWI/SNF components and essential PSPs were maintained in NEAT1-depleted cells, suggesting that SWI/SNF complexes not only facilitate interactions between PSPs, but also recruit PSPs during paraspeckle assembly. SWI/SNF complexes were also required for Satellite III lncRNA-dependent formation of nuclear stress bodies under heat-shock conditions. Our data suggest the existence of a common mechanism underlying the formation of lncRNA-dependent nuclear body architectures in mammalian cells. |Amino Acid Sequence [MESH] |Animals [MESH] |Catalytic Domain [MESH] |Cell Line, Tumor [MESH] |Cell Nucleus/*metabolism [MESH] |Chromatin/*chemistry [MESH] |Chromosomal Proteins, Non-Histone/*chemistry [MESH] |Clustered Regularly Interspaced Short Palindromic Repeats [MESH] |HEK293 Cells [MESH] |HeLa Cells [MESH] |Humans [MESH] |In Situ Hybridization, Fluorescence [MESH] |Mice [MESH] |Molecular Sequence Data [MESH] |Mutation [MESH] |NIH 3T3 Cells [MESH] |RNA, Long Noncoding/*chemistry [MESH] |RNA, Untranslated/*chemistry [MESH]SWI/SNF chromatin-remodeling complexes function in noncoding RNA-depen(epmc).pdf DeepDyve Pubget Overpricing