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10.1073/pnas.1418468112

http://scihub22266oqcxt.onion/10.1073/pnas.1418468112
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C4394297!4394297!25831507
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suck abstract from ncbi


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pmid25831507      Proc+Natl+Acad+Sci+U+S+A 2015 ; 112 (14): 4352-6
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  • Expanded palette of Nano-lanterns for real-time multicolor luminescence imaging #MMPMID25831507
  • Takai A; Nakano M; Saito K; Haruno R; Watanabe TM; Ohyanagi T; Jin T; Okada Y; Nagai T
  • Proc Natl Acad Sci U S A 2015[Apr]; 112 (14): 4352-6 PMID25831507show ga
  • The application of luminescence imaging has been limited mainly by the two drawbacks of luciferases: low brightness and poor color variants. Here, we report the development of cyan and orange luminescent proteins approximately 20 times brighter than the wild-type Renilla luciferase. The color change and enhancement of brightness were both achieved by exploring bioluminescence resonance energy transfer (BRET) from enhanced Renilla luciferase to a fluorescent protein, a technology that we previously reported for the development of the bright yellowish-green luminescent protein Nano-lantern. These cyan and orange Nano-lanterns along with the original yellow Nano-lantern enable monitoring of multiple cellular events, including dynamics of subcellular structures, gene expressions, and functional status, such as intracellular Ca2+ change.
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