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Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Proc+Natl+Acad+Sci+U+S+A 2015 ; 112 (14): 4352-6 Nephropedia Template TP
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Expanded palette of Nano-lanterns for real-time multicolor luminescence imaging #MMPMID25831507
Takai A; Nakano M; Saito K; Haruno R; Watanabe TM; Ohyanagi T; Jin T; Okada Y; Nagai T
Proc Natl Acad Sci U S A 2015[Apr]; 112 (14): 4352-6 PMID25831507show ga
The application of luminescence imaging has been limited mainly by the two drawbacks of luciferases: low brightness and poor color variants. Here, we report the development of cyan and orange luminescent proteins approximately 20 times brighter than the wild-type Renilla luciferase. The color change and enhancement of brightness were both achieved by exploring bioluminescence resonance energy transfer (BRET) from enhanced Renilla luciferase to a fluorescent protein, a technology that we previously reported for the development of the bright yellowish-green luminescent protein Nano-lantern. These cyan and orange Nano-lanterns along with the original yellow Nano-lantern enable monitoring of multiple cellular events, including dynamics of subcellular structures, gene expressions, and functional status, such as intracellular Ca2+ change.