Use my Search Websuite to scan PubMed, PMCentral, Journal Hosts and Journal Archives, FullText. Kick-your-searchterm to multiple Engines kick-your-query now !>

A dictionary by aggregated review articles of nephrology, medicine and the life sciences Your one-stop-run pathway from word to the immediate pdf of peer-reviewed on-topic knowledge.

10.1073/pnas.1417594112 http://scihub22266oqcxt.onion/10.1073/pnas.1417594112 C4394259!4394259 !25831490     free     free     free Warning: file_get_contents(https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pubmed&id=25831490 &cmd=llinks): Failed to open stream: HTTP request failed! HTTP/1.1 429 Too Many Requests in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 215 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 265.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 265.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 265.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Warning: imagejpeg(C:\Inetpub\vhosts\kidney.de\httpdocs\phplern\25831490 .jpg): Failed to open stream: No such file or directory in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 117       Proc+Natl+Acad+Sci+U+S+A 2015 ; 112 (14 ): 4286-91 Nephropedia Template TP {{tp|p=25831490 |t=2015. Human RECQ1 helicase-driven DNA unwinding, annealing, and branch migration: insights from DNA complex structures |pdf=|usr=}}{{25831490 }} gab.com Text Human RECQ1 helicase-driven DNA unwinding, annealing, and branch migration: insights from DNA complex structures JO: Proc Natl Acad Sci U S A http://www.kidney.de/pget.php?&tw=1&pmid=25831490 #FOAvip RecQ helicases are a widely conserved family of ATP-dependent motors with diverse roles in nearly every aspect of bacterial and eukaryotic genome maintenance. However, the physical mechanisms by which RecQ helicases recognize and process specific DNA replication and repair intermediates are largely unknown. Here, we solved crystal structures of the human RECQ1 helicase in complexes with tailed-duplex DNA and ssDNA. The structures map the interactions of the ssDNA tail and the branch point along the helicase and Zn-binding domains, which, together with reported structures of other helicases, define the catalytic stages of helicase action. We also identify a strand-separating pin, which (uniquely in RECQ1) is buttressed by the protein dimer interface. A duplex DNA-binding surface on the C-terminal domain is shown to play a role in DNA unwinding, strand annealing, and Holliday junction (HJ) branch migration. We have combined EM and analytical ultracentrifugation approaches to show that RECQ1 can form what appears to be a flat, homotetrameric complex and propose that RECQ1 tetramers are involved in HJ recognition. This tetrameric arrangement suggests a platform for coordinated activity at the advancing and receding duplexes of an HJ during branch migration. Twit Text FOAVip Human RECQ1 helicase-driven DNA unwinding, annealing, and branch migration: insights from DNA complex structures ????Proc Natl Acad Sci U S A http://www.kidney.de/pget.php?&tw=1&pmid=25831490 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=25831490 #FOAvip English Wikipedia <ref>{{Cite pmid|25831490 }}</ref> Human RECQ1 helicase-driven DNA unwinding, annealing, and branch migration: insights from DNA complex structures #MMPMID25831490 Pike AC ; Gomathinayagam S ; Swuec P ; Berti M ; Zhang Y ; Schnecke C ; Marino F ; von Delft F ; Renault L ; Costa A ; Gileadi O ; Vindigni A Proc Natl Acad Sci U S A 2015[Apr]; 112 (14 ): 4286-91 PMID25831490 show ga RecQ helicases are a widely conserved family of ATP-dependent motors with diverse roles in nearly every aspect of bacterial and eukaryotic genome maintenance. However, the physical mechanisms by which RecQ helicases recognize and process specific DNA replication and repair intermediates are largely unknown. Here, we solved crystal structures of the human RECQ1 helicase in complexes with tailed-duplex DNA and ssDNA. The structures map the interactions of the ssDNA tail and the branch point along the helicase and Zn-binding domains, which, together with reported structures of other helicases, define the catalytic stages of helicase action. We also identify a strand-separating pin, which (uniquely in RECQ1) is buttressed by the protein dimer interface. A duplex DNA-binding surface on the C-terminal domain is shown to play a role in DNA unwinding, strand annealing, and Holliday junction (HJ) branch migration. We have combined EM and analytical ultracentrifugation approaches to show that RECQ1 can form what appears to be a flat, homotetrameric complex and propose that RECQ1 tetramers are involved in HJ recognition. This tetrameric arrangement suggests a platform for coordinated activity at the advancing and receding duplexes of an HJ during branch migration. |Animals [MESH] |Chromatography, Gel [MESH] |Crystallization [MESH] |Crystallography, X-Ray [MESH] |DNA Helicases/*chemistry [MESH] |DNA, Cruciform/physiology [MESH] |DNA, Single-Stranded/chemistry [MESH] |DNA/*chemistry [MESH] |Escherichia coli/metabolism [MESH] |Humans [MESH] |Insecta [MESH] |Molecular Conformation [MESH] |Nucleic Acid Denaturation [MESH] |Nucleotides/chemistry [MESH] |Protein Binding [MESH] |Protein Structure, Tertiary [MESH] |RecQ Helicases/*chemistry [MESH]Human RECQ1 helicase-driven DNA unwinding, annealing, and branch migra(epmc).pdf DeepDyve Pubget Overpricing