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10.1111/j.1582-4934.2011.01386.x http://scihub22266oqcxt.onion/10.1111/j.1582-4934.2011.01386.x C4394234!4394234!21781275     free     free     free       J+Cell+Mol+Med 2011 ; 15 (10): 2269-72 Nephropedia Template TP {{tp|p=21781275|t=2011. Telocytes within human skeletal muscle stem cell niche |pdf=|usr=}}{{21781275}} gab.com Text Telocytes within human skeletal muscle stem cell niche JO: J Cell Mol Med http://www.kidney.de/pget.php?&tw=1&pmid=21781275 #FOAvip Human skeletal muscle tissue displays specific cellular architecture easily damaged during individual existence, requiring multiple resources for regeneration. Congruent with local prerequisites, heterogeneous muscle stem cells (MuSCs) are present in the muscle interstitium. In this study, we aimed to characterize the properties of human muscle interstitial cells that had the characteristic morphology of telocytes (TCs). Immunocytochemistry and immunofluorescence showed that cells with TC morphology stained positive for c-kit/CD117 and VEGF. C-kit positive TCs were separated with magnetic-activated cell sorting, cultured in vitro and expanded for study. These cells exhibited high proliferation capacity (60% expressed endoglin/CD105 and 80% expressed nuclear Ki67). They also exhibited pluripotent capacity limited to Oct4 nuclear staining. In addition, 90% of c-kit positive TCs expressed VEGF. C-kit negative cells in the MuSCs population exhibited fibroblast-like morphology, low trilineage differential potential and negative VEGF staining. These results suggested that c-kit/CD117 positive TCs represented a unique cell type within the MuSC niche. Twit Text FOAVip Telocytes within human skeletal muscle stem cell niche ????J Cell Mol Med http://www.kidney.de/pget.php?&tw=1&pmid=21781275 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=21781275 #FOAvip English Wikipedia <ref>{{Cite pmid|21781275}}</ref> Telocytes within human skeletal muscle stem cell niche #MMPMID21781275 Bojin FM; Gavriliuc OI; Cristea MI; Tanasie G; Tatu CS; Panaitescu C; Paunescu V J Cell Mol Med 2011[Oct]; 15 (10): 2269-72 PMID21781275show ga Human skeletal muscle tissue displays specific cellular architecture easily damaged during individual existence, requiring multiple resources for regeneration. Congruent with local prerequisites, heterogeneous muscle stem cells (MuSCs) are present in the muscle interstitium. In this study, we aimed to characterize the properties of human muscle interstitial cells that had the characteristic morphology of telocytes (TCs). Immunocytochemistry and immunofluorescence showed that cells with TC morphology stained positive for c-kit/CD117 and VEGF. C-kit positive TCs were separated with magnetic-activated cell sorting, cultured in vitro and expanded for study. These cells exhibited high proliferation capacity (60% expressed endoglin/CD105 and 80% expressed nuclear Ki67). They also exhibited pluripotent capacity limited to Oct4 nuclear staining. In addition, 90% of c-kit positive TCs expressed VEGF. C-kit negative cells in the MuSCs population exhibited fibroblast-like morphology, low trilineage differential potential and negative VEGF staining. These results suggested that c-kit/CD117 positive TCs represented a unique cell type within the MuSC niche. äTelocytes within human skeletal muscle stem cell niche (epmc).pdf DeepDyve Pubget Overpricing