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10.1161/HYPERTENSIONAHA.114.05096 http://scihub22266oqcxt.onion/10.1161/HYPERTENSIONAHA.114.05096 C4393363!4393363 !25801876     free     free     free Warning: file_get_contents(https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pubmed&id=25801876 &cmd=llinks): Failed to open stream: HTTP request failed! 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MiR-217 mediates the protective effects of the dopamine D2 receptor on fibrosis in human renal proximal tubule cells |pdf=|usr=}}{{25801876 }} gab.com Text MiR-217 mediates the protective effects of the dopamine D2 receptor on fibrosis in human renal proximal tubule cells JO: Hypertension http://www.kidney.de/pget.php?&tw=1&pmid=25801876 #FOAvip Lack or downregulation of the dopamine D2 receptor (D2R) increases the vulnerability to renal inflammation independent of blood pressure in mice. Common single nucleotide polymorphisms (SNPs) rs6276, 6277, and 1800497 in the human D2R gene are associated with decreased receptor expression/function and hypertension. Human renal proximal tubule cells from subjects carrying these SNPs have decreased D2R expression and increased expression of profibrotic factors and production of extracellular matrix proteins. We tested the hypothesis that the D2R mediates these effects by regulating micro-RNA expression. In cells carrying D2R SNPs, micro-RNAs (miRs)-217, miR-224, miR-335, and miR-1265 were downregulated, whereas miR-1290 was upregulated >4-fold compared with those carrying D2R wild-type alleles. However, only miR-217 was directly regulated by D2R expression. In cells carrying D2R wild-type, miR-217 inhibitor increased the expression of transforming growth factor (TGF)-?1, matrix metalloproteinase 3, fibronectin 1, and collagen 1a, whereas miR-217 mimic had the opposite effect. In cells carrying D2R SNPs, miR-217 mimic also decreased the expression of TGF?1 and its targets. Wnt5a, a miR-217 target, was increased in cells carrying D2R SNPs and decreased by miR-217 mimic but increased by miR-217 inhibitor in both cell types. In cells carrying D2R wild-type, Wnt5a treatment increased TGF?1 while silencing Ror2, a Wnt5a receptor, decreased TGF?1 and blunted the Wnt5a-induced increase in cells carrying D2R wild-type. Our results show that renal proximal tubule cells from subjects carrying D2R SNPs resulting in D2R downregulation have increased TGF?1 that is mediated by decreased regulation of the miR-217-Wnt5a-Ror2 pathway. Twit Text FOAVip MiR-217 mediates the protective effects of the dopamine D2 receptor on fibrosis in human renal proximal tubule cells ????Hypertension http://www.kidney.de/pget.php?&tw=1&pmid=25801876 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=25801876 #FOAvip English Wikipedia <ref>{{Cite pmid|25801876 }}</ref> MiR-217 mediates the protective effects of the dopamine D2 receptor on fibrosis in human renal proximal tubule cells #MMPMID25801876 Han F ; Konkalmatt P ; Chen J ; Gildea J ; Felder RA ; Jose PA ; Armando I Hypertension 2015[May]; 65 (5 ): 1118-25 PMID25801876 show ga Lack or downregulation of the dopamine D2 receptor (D2R) increases the vulnerability to renal inflammation independent of blood pressure in mice. Common single nucleotide polymorphisms (SNPs) rs6276, 6277, and 1800497 in the human D2R gene are associated with decreased receptor expression/function and hypertension. Human renal proximal tubule cells from subjects carrying these SNPs have decreased D2R expression and increased expression of profibrotic factors and production of extracellular matrix proteins. We tested the hypothesis that the D2R mediates these effects by regulating micro-RNA expression. In cells carrying D2R SNPs, micro-RNAs (miRs)-217, miR-224, miR-335, and miR-1265 were downregulated, whereas miR-1290 was upregulated >4-fold compared with those carrying D2R wild-type alleles. However, only miR-217 was directly regulated by D2R expression. In cells carrying D2R wild-type, miR-217 inhibitor increased the expression of transforming growth factor (TGF)-?1, matrix metalloproteinase 3, fibronectin 1, and collagen 1a, whereas miR-217 mimic had the opposite effect. In cells carrying D2R SNPs, miR-217 mimic also decreased the expression of TGF?1 and its targets. Wnt5a, a miR-217 target, was increased in cells carrying D2R SNPs and decreased by miR-217 mimic but increased by miR-217 inhibitor in both cell types. In cells carrying D2R wild-type, Wnt5a treatment increased TGF?1 while silencing Ror2, a Wnt5a receptor, decreased TGF?1 and blunted the Wnt5a-induced increase in cells carrying D2R wild-type. Our results show that renal proximal tubule cells from subjects carrying D2R SNPs resulting in D2R downregulation have increased TGF?1 that is mediated by decreased regulation of the miR-217-Wnt5a-Ror2 pathway. |*Gene Expression Regulation [MESH] |Alleles [MESH] |Cells, Cultured [MESH] |Fibrosis [MESH] |Humans [MESH] |Immunoblotting [MESH] |Kidney Diseases/*genetics/metabolism/pathology [MESH] |Kidney Tubules, Proximal/*metabolism/pathology [MESH] |MicroRNAs/biosynthesis/*genetics [MESH] |Microscopy, Confocal [MESH] |RNA, Messenger/*genetics [MESH] |Real-Time Polymerase Chain Reaction [MESH] |Receptors, Dopamine D2/*genetics/metabolism [MESH]MiR-217 mediates the protective effects of the dopamine D2 receptor on(epmc).pdf DeepDyve Pubget Overpricing