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10.1371/journal.pbio.1002129 http://scihub22266oqcxt.onion/10.1371/journal.pbio.1002129 C4393111!4393111!25860027     free     free     free Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       PLoS+Biol 2015 ; 13 (4): ä Nephropedia Template TP {{tp|p=25860027|t=2015. GSK3?-Dzip1-Rab8 Cascade Regulates Ciliogenesis after Mitosis |pdf=|usr=}}{{25860027}} gab.com Text GSK3 -Dzip1-Rab8 Cascade Regulates Ciliogenesis after Mitosis JO: PLoS Biol http://www.kidney.de/pget.php?&tw=1&pmid=25860027 #FOAvip The primary cilium, which disassembles before mitotic entry and reassembles after mitosis, organizes many signal transduction pathways that are crucial for cell life and individual development. However, how ciliogenesis is regulated during the cell cycle remains largely unknown. Here we show that GSK3?, Dzip1, and Rab8 co-regulate ciliogenesis by promoting the assembly of the ciliary membrane after mitosis. Immunofluorescence and super-resolution microscopy showed that Dzip1 was localized to the periciliary diffusion barrier and enriched at the mother centriole. Knockdown of Dzip1 by short hairpin RNAs led to failed ciliary localization of Rab8, and Rab8 accumulation at the basal body. Dzip1 preferentially bound to Rab8GDP and promoted its dissociation from its inhibitor GDI2 at the pericentriolar region, as demonstrated by sucrose gradient centrifugation of purified basal bodies, immunoprecipitation, and acceptor-bleaching fluorescence resonance energy transfer assays. By means of in vitro phosphorylation, in vivo gel shift, phospho-peptide identification by mass spectrometry, and GST pulldown assays, we demonstrated that Dzip1 was phosphorylated by GSK3? at S520 in G0 phase, which increased its binding to GDI2 to promote the release of Rab8GDP at the cilium base. Moreover, ciliogenesis was inhibited by overexpression of the GSK3?-nonphosphorylatable Dzip1 mutant or by disabling of GSK3? by specific inhibitors or knockout of GSK3? in cells. Collectively, our data reveal a unique cascade consisting of GSK3?, Dzip1, and Rab8 that regulates ciliogenesis after mitosis. Twit Text FOAVip GSK3 -Dzip1-Rab8 Cascade Regulates Ciliogenesis after Mitosis ????PLoS Biol http://www.kidney.de/pget.php?&tw=1&pmid=25860027 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=25860027 #FOAvip English Wikipedia <ref>{{Cite pmid|25860027}}</ref> GSK3?-Dzip1-Rab8 Cascade Regulates Ciliogenesis after Mitosis #MMPMID25860027 Zhang B; Zhang T; Wang G; Wang G; Chi W; Jiang Q; Zhang C PLoS Biol 2015[Apr]; 13 (4): ä PMID25860027show ga The primary cilium, which disassembles before mitotic entry and reassembles after mitosis, organizes many signal transduction pathways that are crucial for cell life and individual development. However, how ciliogenesis is regulated during the cell cycle remains largely unknown. Here we show that GSK3?, Dzip1, and Rab8 co-regulate ciliogenesis by promoting the assembly of the ciliary membrane after mitosis. Immunofluorescence and super-resolution microscopy showed that Dzip1 was localized to the periciliary diffusion barrier and enriched at the mother centriole. Knockdown of Dzip1 by short hairpin RNAs led to failed ciliary localization of Rab8, and Rab8 accumulation at the basal body. Dzip1 preferentially bound to Rab8GDP and promoted its dissociation from its inhibitor GDI2 at the pericentriolar region, as demonstrated by sucrose gradient centrifugation of purified basal bodies, immunoprecipitation, and acceptor-bleaching fluorescence resonance energy transfer assays. By means of in vitro phosphorylation, in vivo gel shift, phospho-peptide identification by mass spectrometry, and GST pulldown assays, we demonstrated that Dzip1 was phosphorylated by GSK3? at S520 in G0 phase, which increased its binding to GDI2 to promote the release of Rab8GDP at the cilium base. Moreover, ciliogenesis was inhibited by overexpression of the GSK3?-nonphosphorylatable Dzip1 mutant or by disabling of GSK3? by specific inhibitors or knockout of GSK3? in cells. Collectively, our data reveal a unique cascade consisting of GSK3?, Dzip1, and Rab8 that regulates ciliogenesis after mitosis. äGSK3?-Dzip1-Rab8 Cascade Regulates Ciliogenesis after Mitosis (epmc).pdf DeepDyve Pubget Overpricing