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10.1002/anie.201409075 http://scihub22266oqcxt.onion/10.1002/anie.201409075 C4392885!4392885!25346227     free     free     free Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 267.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 267.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 267.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Angew+Chem+Int+Ed+Engl 2014 ; 53 (50): 13931-3 Nephropedia Template TP {{tp|p=25346227|t=2014. Over 10 000 peptide identifications from the HeLa proteome using single-shot capillary zone electrophoresis-tandem mass spectrometry |pdf=|usr=}}{{25346227}} gab.com Text Over 10 000 peptide identifications from the HeLa proteome using single-shot capillary zone electrophoresis-tandem mass spectrometry JO: Angew Chem Int Ed Engl http://www.kidney.de/pget.php?&tw=1&pmid=25346227 #FOAvip Capillary zone electrophoresis-tandem mass spectrometry (CZE-MS/MS) has recently attracted attention as a tool for shotgun proteomics. However, its performance for this analysis has fallen far below that of reversed phase liquid chromatography (RPLC)-MS/MS. Here, we report the use of a CZE method with a wide separation window (up to 90 min) and high peak capacity (~300). This method is coupled to an Orbitrap Fusion mass spectrometer via an electro-kinetically pumped sheath flow interface for analysis of complex proteome digests. Single-shot CZE-MS/MS identified over 10 000 peptides and 2 100 proteins from a HeLa cell proteome digest in ~100 min. This performance is nearly an order of magnitude superior to earlier CZE studies and is within a factor of 2 to 4 of state-of-the-art nano ultrahigh pressure LC system. Twit Text FOAVip Over 10 000 peptide identifications from the HeLa proteome using single-shot capillary zone electrophoresis-tandem mass spectrometry ????Angew Chem Int Ed Engl http://www.kidney.de/pget.php?&tw=1&pmid=25346227 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=25346227 #FOAvip English Wikipedia <ref>{{Cite pmid|25346227}}</ref> Over 10 000 peptide identifications from the HeLa proteome using single-shot capillary zone electrophoresis-tandem mass spectrometry #MMPMID25346227 Sun L; Hebert AS; Yan X; Zhao Y; Westphall MS; Rush MJP; Zhu G; Champion MM; Coon JJ; Dovichi NJ Angew Chem Int Ed Engl 2014[Dec]; 53 (50): 13931-3 PMID25346227show ga Capillary zone electrophoresis-tandem mass spectrometry (CZE-MS/MS) has recently attracted attention as a tool for shotgun proteomics. However, its performance for this analysis has fallen far below that of reversed phase liquid chromatography (RPLC)-MS/MS. Here, we report the use of a CZE method with a wide separation window (up to 90 min) and high peak capacity (~300). This method is coupled to an Orbitrap Fusion mass spectrometer via an electro-kinetically pumped sheath flow interface for analysis of complex proteome digests. Single-shot CZE-MS/MS identified over 10 000 peptides and 2 100 proteins from a HeLa cell proteome digest in ~100 min. This performance is nearly an order of magnitude superior to earlier CZE studies and is within a factor of 2 to 4 of state-of-the-art nano ultrahigh pressure LC system. äOver 10 000 peptide identifications from the HeLa proteome using singl(epmc).pdf DeepDyve Pubget Overpricing