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10.1038/nbt.3155 http://scihub22266oqcxt.onion/10.1038/nbt.3155 C4390466!4390466!25690852     free     free     free       Nat+Biotechnol 2015 ; 33 (4): 390-4 Nephropedia Template TP {{tp|p=25690852|t=2015. Inducible in vivo genome editing with CRISPR/Cas9 |pdf=|usr=}}{{25690852}} gab.com Text Inducible in vivo genome editing with CRISPR/Cas9 JO: Nat Biotechnol http://www.kidney.de/pget.php?&tw=1&pmid=25690852 #FOAvip CRISPR/Cas9-based genome editing enables the rapid genetic manipulation of any genomic locus without the need for gene targeting by homologous recombination. Here we describe a conditional transgenic approach that allows temporal control of CRISPR/Cas9 activity for inducible genome editing in adult mice. We show that doxycycline-regulated Cas9 induction enables widespread gene disruption in multiple tissues and that limiting the duration of Cas9 expression or using a Cas9D10A (Cas9n) variant, can regulate the frequency and size of target gene modifications, respectively. Further, we show that the inducible CRISPR (iCRISPR) system can be used effectively to create biallelic mutation in multiple target loci and thus, provides a flexible and fast platform to study loss of function phenotypes in vivo. Twit Text FOAVip Inducible in vivo genome editing with CRISPR/Cas9 ????Nat Biotechnol http://www.kidney.de/pget.php?&tw=1&pmid=25690852 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=25690852 #FOAvip English Wikipedia <ref>{{Cite pmid|25690852}}</ref> Inducible in vivo genome editing with CRISPR/Cas9 #MMPMID25690852 Dow LE; Fisher J; O'Rourke KP; Muley A; Kastenhuber ER; Livshits G; Tschaharganeh DF; Socci ND; Lowe SW Nat Biotechnol 2015[Apr]; 33 (4): 390-4 PMID25690852show ga CRISPR/Cas9-based genome editing enables the rapid genetic manipulation of any genomic locus without the need for gene targeting by homologous recombination. Here we describe a conditional transgenic approach that allows temporal control of CRISPR/Cas9 activity for inducible genome editing in adult mice. We show that doxycycline-regulated Cas9 induction enables widespread gene disruption in multiple tissues and that limiting the duration of Cas9 expression or using a Cas9D10A (Cas9n) variant, can regulate the frequency and size of target gene modifications, respectively. Further, we show that the inducible CRISPR (iCRISPR) system can be used effectively to create biallelic mutation in multiple target loci and thus, provides a flexible and fast platform to study loss of function phenotypes in vivo. äInducible in vivo genome editing with CRISPR/Cas9 (epmc).pdf DeepDyve Pubget Overpricing