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10.1074/mcp.M115.048272 http://scihub22266oqcxt.onion/10.1074/mcp.M115.048272 C4390274!4390274 !25670804     free     free     free Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 265.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 265.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Warning: imagejpeg(C:\Inetpub\vhosts\kidney.de\httpdocs\phplern\25670804 .jpg): Failed to open stream: No such file or directory in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 117       Mol+Cell+Proteomics 2015 ; 14 (4 ): 974-88 Nephropedia Template TP {{tp|p=25670804 |t=2015. Unravelling glucan recognition systems by glycome microarrays using the designer approach and mass spectrometry |pdf=|usr=}}{{25670804 }} gab.com Text Unravelling glucan recognition systems by glycome microarrays using the designer approach and mass spectrometry JO: Mol Cell Proteomics http://www.kidney.de/pget.php?&tw=1&pmid=25670804 #FOAvip Glucans are polymers of d-glucose with differing linkages in linear or branched sequences. They are constituents of microbial and plant cell-walls and involved in important bio-recognition processes, including immunomodulation, anticancer activities, pathogen virulence, and plant cell-wall biodegradation. Translational possibilities for these activities in medicine and biotechnology are considerable. High-throughput micro-methods are needed to screen proteins for recognition of specific glucan sequences as a lead to structure-function studies and their exploitation. We describe construction of a "glucome" microarray, the first sequence-defined glycome-scale microarray, using a "designer" approach from targeted ligand-bearing glucans in conjunction with a novel high-sensitivity mass spectrometric sequencing method, as a screening tool to assign glucan recognition motifs. The glucome microarray comprises 153 oligosaccharide probes with high purity, representing major sequences in glucans. Negative-ion electrospray tandem mass spectrometry with collision-induced dissociation was used for complete linkage analysis of gluco-oligosaccharides in linear "homo" and "hetero" and branched sequences. The system is validated using antibodies and carbohydrate-binding modules known to target ?- or ?-glucans in different biological contexts, extending knowledge on their specificities, and applied to reveal new information on glucan recognition by two signaling molecules of the immune system against pathogens: Dectin-1 and DC-SIGN. The sequencing of the glucan oligosaccharides by the MS method and their interrogation on the microarrays provides detailed information on linkage, sequence and chain length requirements of glucan-recognizing proteins, and are a sensitive means of revealing unsuspected sequences in the polysaccharides. Twit Text FOAVip Unravelling glucan recognition systems by glycome microarrays using the designer approach and mass spectrometry ????Mol Cell Proteomics http://www.kidney.de/pget.php?&tw=1&pmid=25670804 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=25670804 #FOAvip English Wikipedia <ref>{{Cite pmid|25670804 }}</ref> Unravelling glucan recognition systems by glycome microarrays using the designer approach and mass spectrometry #MMPMID25670804 Palma AS ; Liu Y ; Zhang H ; Zhang Y ; McCleary BV ; Yu G ; Huang Q ; Guidolin LS ; Ciocchini AE ; Torosantucci A ; Wang D ; Carvalho AL ; Fontes CM ; Mulloy B ; Childs RA ; Feizi T ; Chai W Mol Cell Proteomics 2015[Apr]; 14 (4 ): 974-88 PMID25670804 show ga Glucans are polymers of d-glucose with differing linkages in linear or branched sequences. They are constituents of microbial and plant cell-walls and involved in important bio-recognition processes, including immunomodulation, anticancer activities, pathogen virulence, and plant cell-wall biodegradation. Translational possibilities for these activities in medicine and biotechnology are considerable. High-throughput micro-methods are needed to screen proteins for recognition of specific glucan sequences as a lead to structure-function studies and their exploitation. We describe construction of a "glucome" microarray, the first sequence-defined glycome-scale microarray, using a "designer" approach from targeted ligand-bearing glucans in conjunction with a novel high-sensitivity mass spectrometric sequencing method, as a screening tool to assign glucan recognition motifs. The glucome microarray comprises 153 oligosaccharide probes with high purity, representing major sequences in glucans. Negative-ion electrospray tandem mass spectrometry with collision-induced dissociation was used for complete linkage analysis of gluco-oligosaccharides in linear "homo" and "hetero" and branched sequences. The system is validated using antibodies and carbohydrate-binding modules known to target ?- or ?-glucans in different biological contexts, extending knowledge on their specificities, and applied to reveal new information on glucan recognition by two signaling molecules of the immune system against pathogens: Dectin-1 and DC-SIGN. The sequencing of the glucan oligosaccharides by the MS method and their interrogation on the microarrays provides detailed information on linkage, sequence and chain length requirements of glucan-recognizing proteins, and are a sensitive means of revealing unsuspected sequences in the polysaccharides. |Animals [MESH] |Antibodies/metabolism [MESH] |Carbohydrate Sequence [MESH] |Cell Adhesion Molecules/metabolism [MESH] |Glucans/*metabolism [MESH] |Immune System/metabolism [MESH] |Lectins, C-Type/metabolism [MESH] |Ligands [MESH] |Mice [MESH] |Oligosaccharides/chemistry/metabolism [MESH] |Protein Array Analysis/*methods [MESH] |Protein Binding [MESH] |Proteome/*metabolism [MESH] |Receptors, Cell Surface/metabolism [MESH] |Reproducibility of Results [MESH] |Signal Transduction [MESH] |Spectrometry, Mass, Electrospray Ionization/*methods [MESH]Unravelling glucan recognition systems by glycome microarrays using th(epmc).pdf DeepDyve Pubget Overpricing