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10.4161/auto.26856 http://scihub22266oqcxt.onion/10.4161/auto.26856 C4389869!4389869!24270884     free     free     free Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Autophagy 2014 ; 10 (1): 155-64 Nephropedia Template TP {{tp|p=24270884|t=2014. Estimating the size and number of autophagic bodies by electron microscopy |pdf=|usr=}}{{24270884}} gab.com Text Estimating the size and number of autophagic bodies by electron microscopy JO: Autophagy http://www.kidney.de/pget.php?&tw=1&pmid=24270884 #FOAvip Much recent and ongoing research is focused on understanding the mechanisms and regulation of autophagy, a cellular self-degradation pathway with many links to human health. Although many assays exist to measure the total magnitude of autophagy, electron microscopy remains the tool of choice for the determination of the size and the number of autophagosomes formed in a given mutant or under given induction conditions. Here we present a detailed protocol for measuring autophagic bodies in the yeast Saccharomyces cerevisiae by electron microscopy. Furthermore, we present an improved mathematical method for estimating body size and a new method for estimating body number. Finally, we include a discussion of the merits and limitations of these methods and an example of their application to autophagic bodies formed in the ume6? strain. Twit Text FOAVip Estimating the size and number of autophagic bodies by electron microscopy ????Autophagy http://www.kidney.de/pget.php?&tw=1&pmid=24270884 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=24270884 #FOAvip English Wikipedia <ref>{{Cite pmid|24270884}}</ref> Estimating the size and number of autophagic bodies by electron microscopy #MMPMID24270884 Backues SK; Chen D; Ruan J; Xie Z; Klionsky DJ Autophagy 2014[Jan]; 10 (1): 155-64 PMID24270884show ga Much recent and ongoing research is focused on understanding the mechanisms and regulation of autophagy, a cellular self-degradation pathway with many links to human health. Although many assays exist to measure the total magnitude of autophagy, electron microscopy remains the tool of choice for the determination of the size and the number of autophagosomes formed in a given mutant or under given induction conditions. Here we present a detailed protocol for measuring autophagic bodies in the yeast Saccharomyces cerevisiae by electron microscopy. Furthermore, we present an improved mathematical method for estimating body size and a new method for estimating body number. Finally, we include a discussion of the merits and limitations of these methods and an example of their application to autophagic bodies formed in the ume6? strain. äEstimating the size and number of autophagic bodies by electron micros(epmc).pdf DeepDyve Pubget Overpricing