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10.1186/s12885-015-1196-y http://scihub22266oqcxt.onion/10.1186/s12885-015-1196-y C4389787!4389787!25879842     free     free     free Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       BMC+Cancer 2015 ; 15 (ä): ä Nephropedia Template TP {{tp|p=25879842|t=2015. The role of multipotent cancer associated fibroblasts in hepatocarcinogenesis |pdf=|usr=}}{{25879842}} gab.com Text The role of multipotent cancer associated fibroblasts in hepatocarcinogenesis JO: BMC Cancer http://www.kidney.de/pget.php?&tw=1&pmid=25879842 #FOAvip Background: The presence of tumor supporting cells in various cancer, including in hepatocellular carcinoma (HCC), has become an important target in the study of carcinogenesis. The cancer-associated fibroblast (CAF), one of the most important cellular components in the cancer stroma, might contribute to the progression of the disease due to its plasticity, a behavior of the stem cells. In this study, we investigate the significance of the CAF and its role in the HCC progression and metastasis. Methods: Primary CAF and non-tumoral fibroblast (NTF) from nine paired HCC and distant non-tumoral liver tissues were isolated and cultured. The cells were characterized by flow cytometry, RT-PCR, anchorage-independent assay and in vitro cells directed trans-differentiation. Co-culture study was performed in Transwell system and xenograft assay was performed in immunodeficient mice. Results: CAF and NTF were positive for CD90, CD44, ?SMA, and vimentin and negative for CD34, CD45, CD117, and CD133. When stimulated, they showed the potential to differentiate into adipocytes, osteoblasts, and pancreatic cells. When co-cultured with human HCC cell lines, CAF up-regulated gene expressions of TGFB1 and FAP of HuH-7 and JHH-6 while NTF did not induced either of the genes. Xenograft assay showed that the CAF had the capacity to enter into circulation as confirmed by RT-PCR and DNA sequencing. Conclusion: Our data provides evidence of the plasticity of the CAF and the NTF as stem cells in the process of hepatocarcinogenesis and metastasis. These cells mutually interacts with HCC cells. Their trans-differentiation flexibility may induce a switch from normal to cancerous microenvironment. Electronic supplementary material: The online version of this article (doi:10.1186/s12885-015-1196-y) contains supplementary material, which is available to authorized users. Twit Text FOAVip The role of multipotent cancer associated fibroblasts in hepatocarcinogenesis ????BMC Cancer http://www.kidney.de/pget.php?&tw=1&pmid=25879842 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=25879842 #FOAvip English Wikipedia <ref>{{Cite pmid|25879842}}</ref> The role of multipotent cancer associated fibroblasts in hepatocarcinogenesis #MMPMID25879842 Sukowati CHC; Anfuso B; Crocé LS; Tiribelli C BMC Cancer 2015[]; 15 (ä): ä PMID25879842show ga Background: The presence of tumor supporting cells in various cancer, including in hepatocellular carcinoma (HCC), has become an important target in the study of carcinogenesis. The cancer-associated fibroblast (CAF), one of the most important cellular components in the cancer stroma, might contribute to the progression of the disease due to its plasticity, a behavior of the stem cells. In this study, we investigate the significance of the CAF and its role in the HCC progression and metastasis. Methods: Primary CAF and non-tumoral fibroblast (NTF) from nine paired HCC and distant non-tumoral liver tissues were isolated and cultured. The cells were characterized by flow cytometry, RT-PCR, anchorage-independent assay and in vitro cells directed trans-differentiation. Co-culture study was performed in Transwell system and xenograft assay was performed in immunodeficient mice. Results: CAF and NTF were positive for CD90, CD44, ?SMA, and vimentin and negative for CD34, CD45, CD117, and CD133. When stimulated, they showed the potential to differentiate into adipocytes, osteoblasts, and pancreatic cells. When co-cultured with human HCC cell lines, CAF up-regulated gene expressions of TGFB1 and FAP of HuH-7 and JHH-6 while NTF did not induced either of the genes. Xenograft assay showed that the CAF had the capacity to enter into circulation as confirmed by RT-PCR and DNA sequencing. Conclusion: Our data provides evidence of the plasticity of the CAF and the NTF as stem cells in the process of hepatocarcinogenesis and metastasis. These cells mutually interacts with HCC cells. Their trans-differentiation flexibility may induce a switch from normal to cancerous microenvironment. Electronic supplementary material: The online version of this article (doi:10.1186/s12885-015-1196-y) contains supplementary material, which is available to authorized users. äThe role of multipotent cancer associated fibroblasts in hepatocarcino(epmc).pdf DeepDyve Pubget Overpricing