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10.1371/journal.pone.0123046 http://scihub22266oqcxt.onion/10.1371/journal.pone.0123046 C4388650!4388650 !25849590     free     free     free Deprecated: Implicit conversion from float 227.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 227.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 227.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 227.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 227.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 227.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 227.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 261.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 261.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 261.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Warning: imagejpeg(C:\Inetpub\vhosts\kidney.de\httpdocs\phplern\25849590 .jpg): Failed to open stream: No such file or directory in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 117       PLoS+One 2015 ; 10 (4 ): e0123046 Nephropedia Template TP {{tp|p=25849590 |t=2015. TGF-?1-mediated differentiation of fibroblasts is associated with increased mitochondrial content and cellular respiration |pdf=|usr=}}{{25849590 }} gab.com Text TGF- 1-mediated differentiation of fibroblasts is associated with increased mitochondrial content and cellular respiration JO: PLoS One http://www.kidney.de/pget.php?&tw=1&pmid=25849590 #FOAvip OBJECTIVS: Cytokine-dependent activation of fibroblasts to myofibroblasts, a key event in fibrosis, is accompanied by phenotypic changes with increased secretory and contractile properties dependent on increased energy utilization, yet changes in the energetic profile of these cells are not fully described. We hypothesize that the TGF-?1-mediated transformation of myofibroblasts is associated with an increase in mitochondrial content and function when compared to naive fibroblasts. METHODS: Cultured NIH/3T3 mouse fibroblasts treated with TGF-?1, a profibrotic cytokine, or vehicle were assessed for transformation to myofibroblasts (appearance of ?-smooth muscle actin [?-SMA] stress fibers) and associated changes in mitochondrial content and functions using laser confocal microscopy, Seahorse respirometry, multi-well plate reader and biochemical protocols. Expression of mitochondrial-specific proteins was determined using western blotting, and the mitochondrial DNA quantified using Mitochondrial DNA isolation kit. RESULTS: Treatment with TGF-?1 (5 ng/mL) induced transformation of naive fibroblasts into myofibroblasts with a threefold increase in the expression of ?-SMA (6.85 ± 0.27 RU) compared to cells not treated with TGF-?1 (2.52 ± 0.11 RU). TGF-?1 exposure increased the number of mitochondria in the cells, as monitored by membrane potential sensitive dye tetramethylrhodamine, and expression of mitochondria-specific proteins; voltage-dependent anion channels (0.54 ± 0.05 vs. 0.23 ± 0.05 RU) and adenine nucleotide transporter (0.61 ± 0.11 vs. 0.22 ± 0.05 RU), as well as mitochondrial DNA content (530 ± 12 ?g DNA/106 cells vs. 307 ± 9 ?g DNA/106 cells in control). TGF-?1 treatment was associated with an increase in mitochondrial function with a twofold increase in baseline oxygen consumption rate (2.25 ± 0.03 vs. 1.13 ± 0.1 nmol O2/min/106 cells) and FCCP-induced mitochondrial respiration (2.87 ± 0.03 vs. 1.46 ± 0.15 nmol O2/min/106 cells). CONCLUSIONS: TGF-?1 induced differentiation of fibroblasts is accompanied by energetic remodeling of myofibroblasts with an increase in mitochondrial respiration and mitochondrial content. Twit Text FOAVip TGF- 1-mediated differentiation of fibroblasts is associated with increased mitochondrial content and cellular respiration ????PLoS One http://www.kidney.de/pget.php?&tw=1&pmid=25849590 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=25849590 #FOAvip English Wikipedia <ref>{{Cite pmid|25849590 }}</ref> TGF-?1-mediated differentiation of fibroblasts is associated with increased mitochondrial content and cellular respiration #MMPMID25849590 Negmadjanov U ; Godic Z ; Rizvi F ; Emelyanova L ; Ross G ; Richards J ; Holmuhamedov EL ; Jahangir A PLoS One 2015[]; 10 (4 ): e0123046 PMID25849590 show ga OBJECTIVS: Cytokine-dependent activation of fibroblasts to myofibroblasts, a key event in fibrosis, is accompanied by phenotypic changes with increased secretory and contractile properties dependent on increased energy utilization, yet changes in the energetic profile of these cells are not fully described. We hypothesize that the TGF-?1-mediated transformation of myofibroblasts is associated with an increase in mitochondrial content and function when compared to naive fibroblasts. METHODS: Cultured NIH/3T3 mouse fibroblasts treated with TGF-?1, a profibrotic cytokine, or vehicle were assessed for transformation to myofibroblasts (appearance of ?-smooth muscle actin [?-SMA] stress fibers) and associated changes in mitochondrial content and functions using laser confocal microscopy, Seahorse respirometry, multi-well plate reader and biochemical protocols. Expression of mitochondrial-specific proteins was determined using western blotting, and the mitochondrial DNA quantified using Mitochondrial DNA isolation kit. RESULTS: Treatment with TGF-?1 (5 ng/mL) induced transformation of naive fibroblasts into myofibroblasts with a threefold increase in the expression of ?-SMA (6.85 ± 0.27 RU) compared to cells not treated with TGF-?1 (2.52 ± 0.11 RU). TGF-?1 exposure increased the number of mitochondria in the cells, as monitored by membrane potential sensitive dye tetramethylrhodamine, and expression of mitochondria-specific proteins; voltage-dependent anion channels (0.54 ± 0.05 vs. 0.23 ± 0.05 RU) and adenine nucleotide transporter (0.61 ± 0.11 vs. 0.22 ± 0.05 RU), as well as mitochondrial DNA content (530 ± 12 ?g DNA/106 cells vs. 307 ± 9 ?g DNA/106 cells in control). TGF-?1 treatment was associated with an increase in mitochondrial function with a twofold increase in baseline oxygen consumption rate (2.25 ± 0.03 vs. 1.13 ± 0.1 nmol O2/min/106 cells) and FCCP-induced mitochondrial respiration (2.87 ± 0.03 vs. 1.46 ± 0.15 nmol O2/min/106 cells). CONCLUSIONS: TGF-?1 induced differentiation of fibroblasts is accompanied by energetic remodeling of myofibroblasts with an increase in mitochondrial respiration and mitochondrial content. |Animals [MESH] |Blotting, Western [MESH] |Cell Differentiation/*drug effects [MESH] |Cell Respiration/*physiology [MESH] |Cells, Cultured [MESH] |Fibroblasts/*cytology/drug effects/metabolism [MESH] |Immunoenzyme Techniques [MESH] |Mice [MESH] |Mitochondria/drug effects/metabolism/*pathology [MESH] |Mitochondrial Proteins/*metabolism [MESH] |NIH 3T3 Cells [MESH] |RNA, Messenger/metabolism [MESH]TGF-?1-mediated differentiation of fibroblasts is associated with incr(epmc).pdf DeepDyve Pubget Overpricing