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10.1038/nsmb.2979 http://scihub22266oqcxt.onion/10.1038/nsmb.2979 C4385408!4385408!25730778     free     free     free Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Nat+Struct+Mol+Biol 2015 ; 22 (4): 328-35 Nephropedia Template TP {{tp|p=25730778|t=2015. Small-RNA loading licenses Argonaute for assembly into a transcriptional silencing complex |pdf=|usr=}}{{25730778}} gab.com Text Small-RNA loading licenses Argonaute for assembly into a transcriptional silencing complex JO: Nat Struct Mol Biol http://www.kidney.de/pget.php?&tw=1&pmid=25730778 #FOAvip Argonautes and their small-RNA cofactors form the core effectors of ancient and diverse gene-silencing mechanisms whose roles include regulation of gene expression and defense against foreign genetic elements. Although Argonautes generally act within multisubunit complexes, what governs their assembly into these machineries is not well defined. Here, we show that loading of small RNAs onto Argonaute is a checkpoint for Argonaute?s association with conserved GW-protein components of silencing complexes. We demonstrate that the Argonaute small interfering RNA chaperone (ARC) complex mediates loading of small RNAs onto Ago1 in Schizosaccharomyces pombe and that deletion of its subunits, or mutations in Ago1 that prevent small-RNA loading, abolish the assembly of the GW protein?containing RNA-induced transcriptional silencing (RITS) complex. Our studies uncover a mechanism that ensures that Argonaute loading precedes RITS assembly and thereby averts the formation of inert and potentially deleterious complexes. Twit Text FOAVip Small-RNA loading licenses Argonaute for assembly into a transcriptional silencing complex ????Nat Struct Mol Biol http://www.kidney.de/pget.php?&tw=1&pmid=25730778 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=25730778 #FOAvip English Wikipedia <ref>{{Cite pmid|25730778}}</ref> Small-RNA loading licenses Argonaute for assembly into a transcriptional silencing complex #MMPMID25730778 Holoch D; Moazed D Nat Struct Mol Biol 2015[Apr]; 22 (4): 328-35 PMID25730778show ga Argonautes and their small-RNA cofactors form the core effectors of ancient and diverse gene-silencing mechanisms whose roles include regulation of gene expression and defense against foreign genetic elements. Although Argonautes generally act within multisubunit complexes, what governs their assembly into these machineries is not well defined. Here, we show that loading of small RNAs onto Argonaute is a checkpoint for Argonaute?s association with conserved GW-protein components of silencing complexes. We demonstrate that the Argonaute small interfering RNA chaperone (ARC) complex mediates loading of small RNAs onto Ago1 in Schizosaccharomyces pombe and that deletion of its subunits, or mutations in Ago1 that prevent small-RNA loading, abolish the assembly of the GW protein?containing RNA-induced transcriptional silencing (RITS) complex. Our studies uncover a mechanism that ensures that Argonaute loading precedes RITS assembly and thereby averts the formation of inert and potentially deleterious complexes. äSmall-RNA loading licenses Argonaute for assembly into a transcription(epmc).pdf DeepDyve Pubget Overpricing