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10.3892/ijo.2015.2911 http://scihub22266oqcxt.onion/10.3892/ijo.2015.2911 C4383027!4383027 !25738332     free     free     free Warning: file_get_contents(https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pubmed&id=25738332 &cmd=llinks): Failed to open stream: HTTP request failed! 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Lithium chloride induces mesenchymal?to?epithelial reverting transition in primary colon cancer cell cultures |pdf=|usr=}}{{25738332 }} gab.com Text Lithium chloride induces mesenchymal to epithelial reverting transition in primary colon cancer cell cultures JO: Int J Oncol http://www.kidney.de/pget.php?&tw=1&pmid=25738332 #FOAvip Epithelial?to?mesenchymal transition (EMT) confers stem cell?like phenotype and more motile properties to carcinoma cells. During EMT, the expression of E?cadherin decreases, resulting in loss of cell?cell adhesion and increased migration. Expression of Twist1 and other pleiotropic transcription factors, such as Snail, is known to activate EMT. We established primary colon cancer cell cultures from samples of operated patients and validated cultures by cytogenetic and molecular biology approaches. Western blot assay, quantitative real?time PCR and immunofluorescence were performed to investigate the expression of E?cadherin, vimentin, ??catenin, cytokeratin?20 and ?18, Twist1, Snail, CD44, cyclooxygenase?2 (COX2), Sox2, Oct4 and Nanog. Moreover, cell differentiation was induced by incubation with LiCl?containing medium for 10 days. We observed that these primary colorectal cancer (CRC) cells lost expression of the E?cadherin epithelial marker, which was instead expressed in cancer and normal colon mucosa of the same patient, while overexpressed vimentin (mesenchymal marker), Twist1, Snail (EMT markers) and COX2. Cytokeratin?18 was expressed both in tissues and cell cultures. Expression of stem cell markers, such as CD44, Oct4 and Nanog, were also observed. Following differentiation with the glycogen synthase kinase 3? (GSK3?) inhibitor LiCl, the cells began to express E?cadherin and, at once, Twist1 and Snail expression was strongly downregulated, suggesting a MET?reverting process. In conclusion, we established primary colon mesenchymal cancer cell cultures expressing mesenchymal and epithelial biomarkers together with high level of EMT transcription factors. We propose that they could represent a good model for studying EMT and its reverting mechanism, the mesenchymal?to?epithelial transition (MET). Our observation indicates that LiCl, a GSK3? inhibitor, induces MET in vitro, suggesting that LiCl and GSK3? could represent, respectively, interesting drug, and target for CRC therapy. Twit Text FOAVip Lithium chloride induces mesenchymal to epithelial reverting transition in primary colon cancer cell cultures ????Int J Oncol http://www.kidney.de/pget.php?&tw=1&pmid=25738332 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=25738332 #FOAvip English Wikipedia <ref>{{Cite pmid|25738332 }}</ref> Lithium chloride induces mesenchymal?to?epithelial reverting transition in primary colon cancer cell cultures #MMPMID25738332 Costabile V ; Duraturo F ; Delrio P ; Rega D ; Pace U ; Liccardo R ; Rossi GB ; Genesio R ; Nitsch L ; Izzo P ; De Rosa M Int J Oncol 2015[May]; 46 (5 ): 1913-23 PMID25738332 show ga Epithelial?to?mesenchymal transition (EMT) confers stem cell?like phenotype and more motile properties to carcinoma cells. During EMT, the expression of E?cadherin decreases, resulting in loss of cell?cell adhesion and increased migration. Expression of Twist1 and other pleiotropic transcription factors, such as Snail, is known to activate EMT. We established primary colon cancer cell cultures from samples of operated patients and validated cultures by cytogenetic and molecular biology approaches. Western blot assay, quantitative real?time PCR and immunofluorescence were performed to investigate the expression of E?cadherin, vimentin, ??catenin, cytokeratin?20 and ?18, Twist1, Snail, CD44, cyclooxygenase?2 (COX2), Sox2, Oct4 and Nanog. Moreover, cell differentiation was induced by incubation with LiCl?containing medium for 10 days. We observed that these primary colorectal cancer (CRC) cells lost expression of the E?cadherin epithelial marker, which was instead expressed in cancer and normal colon mucosa of the same patient, while overexpressed vimentin (mesenchymal marker), Twist1, Snail (EMT markers) and COX2. Cytokeratin?18 was expressed both in tissues and cell cultures. Expression of stem cell markers, such as CD44, Oct4 and Nanog, were also observed. Following differentiation with the glycogen synthase kinase 3? (GSK3?) inhibitor LiCl, the cells began to express E?cadherin and, at once, Twist1 and Snail expression was strongly downregulated, suggesting a MET?reverting process. In conclusion, we established primary colon mesenchymal cancer cell cultures expressing mesenchymal and epithelial biomarkers together with high level of EMT transcription factors. We propose that they could represent a good model for studying EMT and its reverting mechanism, the mesenchymal?to?epithelial transition (MET). Our observation indicates that LiCl, a GSK3? inhibitor, induces MET in vitro, suggesting that LiCl and GSK3? could represent, respectively, interesting drug, and target for CRC therapy. |Adjuvants, Immunologic/*pharmacology [MESH] |Biomarkers, Tumor/metabolism [MESH] |Blotting, Western [MESH] |Cell Differentiation/drug effects [MESH] |Colonic Neoplasms/*drug therapy/metabolism [MESH] |Epithelial-Mesenchymal Transition/drug effects [MESH] |Fluorescent Antibody Technique [MESH] |Humans [MESH] |Lithium Chloride/*pharmacology [MESH] |Neoplasm Proteins/metabolism [MESH] |Real-Time Polymerase Chain Reaction [MESH]Lithium chloride induces mesenchymal?to?epithelial reverting transitio(epmc).pdf DeepDyve Pubget Overpricing