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10.1093/bioinformatics/btu743 http://scihub22266oqcxt.onion/10.1093/bioinformatics/btu743 C4382898!4382898!25414360     free     free     free Deprecated: Implicit conversion from float 213.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 213.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 213.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 213.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 213.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 213.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Bioinformatics 2015 ; 31 (7): 1120-3 Nephropedia Template TP {{tp|p=25414360|t=2015. CRISPRdirect: software for designing CRISPR/Cas guide RNA with reduced off-target sites |pdf=|usr=}}{{25414360}} gab.com Text CRISPRdirect: software for designing CRISPR/Cas guide RNA with reduced off-target sites JO: Bioinformatics http://www.kidney.de/pget.php?&tw=1&pmid=25414360 #FOAvip Summary: CRISPRdirect is a simple and functional web server for selecting rational CRISPR/Cas targets from an input sequence. The CRISPR/Cas system is a promising technique for genome engineering which allows target-specific cleavage of genomic DNA guided by Cas9 nuclease in complex with a guide RNA (gRNA), that complementarily binds to a ?20 nt targeted sequence. The target sequence requirements are twofold. First, the 5?-NGG protospacer adjacent motif (PAM) sequence must be located adjacent to the target sequence. Second, the target sequence should be specific within the entire genome in order to avoid off-target editing. CRISPRdirect enables users to easily select rational target sequences with minimized off-target sites by performing exhaustive searches against genomic sequences. The server currently incorporates the genomic sequences of human, mouse, rat, marmoset, pig, chicken, frog, zebrafish, Ciona, fruit fly, silkworm, Caenorhabditis elegans, Arabidopsis, rice, Sorghum and budding yeast.Availability: Freely available at http://crispr.dbcls.jp/.Contact:y-naito@dbcls.rois.ac.jpSupplementary information:Supplementary data are available at Bioinformatics online. Twit Text FOAVip CRISPRdirect: software for designing CRISPR/Cas guide RNA with reduced off-target sites ????Bioinformatics http://www.kidney.de/pget.php?&tw=1&pmid=25414360 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=25414360 #FOAvip English Wikipedia <ref>{{Cite pmid|25414360}}</ref> CRISPRdirect: software for designing CRISPR/Cas guide RNA with reduced off-target sites #MMPMID25414360 Naito Y; Hino K; Bono H; Ui-Tei K Bioinformatics 2015[Apr]; 31 (7): 1120-3 PMID25414360show ga Summary: CRISPRdirect is a simple and functional web server for selecting rational CRISPR/Cas targets from an input sequence. The CRISPR/Cas system is a promising technique for genome engineering which allows target-specific cleavage of genomic DNA guided by Cas9 nuclease in complex with a guide RNA (gRNA), that complementarily binds to a ?20 nt targeted sequence. The target sequence requirements are twofold. First, the 5?-NGG protospacer adjacent motif (PAM) sequence must be located adjacent to the target sequence. Second, the target sequence should be specific within the entire genome in order to avoid off-target editing. CRISPRdirect enables users to easily select rational target sequences with minimized off-target sites by performing exhaustive searches against genomic sequences. The server currently incorporates the genomic sequences of human, mouse, rat, marmoset, pig, chicken, frog, zebrafish, Ciona, fruit fly, silkworm, Caenorhabditis elegans, Arabidopsis, rice, Sorghum and budding yeast.Availability: Freely available at http://crispr.dbcls.jp/.Contact:y-naito@dbcls.rois.ac.jpSupplementary information:Supplementary data are available at Bioinformatics online. äCRISPRdirect: software for designing CRISPR/Cas guide RNA with reduced(epmc).pdf DeepDyve Pubget Overpricing