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10.1038/nmeth.3286 http://scihub22266oqcxt.onion/10.1038/nmeth.3286 C4382417!4382417 !25707030     free     free     free Warning: file_get_contents(https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pubmed&id=25707030 &cmd=llinks): Failed to open stream: HTTP request failed! HTTP/1.1 429 Too Many Requests in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 215 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 267.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 267.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Warning: imagejpeg(C:\Inetpub\vhosts\kidney.de\httpdocs\phplern\25707030 .jpg): Failed to open stream: No such file or directory in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 117       Nat+Methods 2015 ; 12 (4 ): 361-365 Nephropedia Template TP {{tp|p=25707030 |t=2015. Atomic-accuracy models from 4 5-Å cryo-electron microscopy data with density-guided iterative local refinement |pdf=|usr=}}{{25707030 }} gab.com Text Atomic-accuracy models from 4 5-Å cryo-electron microscopy data with density-guided iterative local refinement JO: Nat Methods http://www.kidney.de/pget.php?&tw=1&pmid=25707030 #FOAvip We describe a general approach for refining protein structure models on the basis of cryo-electron microscopy maps with near-atomic resolution. The method integrates Monte Carlo sampling with local density-guided optimization, Rosetta all-atom refinement and real-space B-factor fitting. In tests on experimental maps of three different systems with 4.5-Å resolution or better, the method consistently produced models with atomic-level accuracy largely independently of starting-model quality, and it outperformed the molecular dynamics-based MDFF method. Cross-validated model quality statistics correlated with model accuracy over the three test systems. Twit Text FOAVip Atomic-accuracy models from 4 5-Å cryo-electron microscopy data with density-guided iterative local refinement ????Nat Methods http://www.kidney.de/pget.php?&tw=1&pmid=25707030 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=25707030 #FOAvip English Wikipedia <ref>{{Cite pmid|25707030 }}</ref> Atomic-accuracy models from 4 5-Å cryo-electron microscopy data with density-guided iterative local refinement #MMPMID25707030 DiMaio F ; Song Y ; Li X ; Brunner MJ ; Xu C ; Conticello V ; Egelman E ; Marlovits T ; Cheng Y ; Baker D Nat Methods 2015[Apr]; 12 (4 ): 361-365 PMID25707030 show ga We describe a general approach for refining protein structure models on the basis of cryo-electron microscopy maps with near-atomic resolution. The method integrates Monte Carlo sampling with local density-guided optimization, Rosetta all-atom refinement and real-space B-factor fitting. In tests on experimental maps of three different systems with 4.5-Å resolution or better, the method consistently produced models with atomic-level accuracy largely independently of starting-model quality, and it outperformed the molecular dynamics-based MDFF method. Cross-validated model quality statistics correlated with model accuracy over the three test systems. |*Cryoelectron Microscopy [MESH] |*Models, Molecular [MESH] |Chemistry, Physical/*methods [MESH] |Monte Carlo Method [MESH]Atomic-accuracy models from 4 5-Å cryo-electron microscopy data with (epmc).pdf DeepDyve Pubget Overpricing