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10.1093/nar/gku1347 http://scihub22266oqcxt.onion/10.1093/nar/gku1347 C4381045!4381045 !25539918     free     free     free Warning: file_get_contents(https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pubmed&id=25539918 &cmd=llinks): Failed to open stream: HTTP request failed! 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Immediate chromatin immunoprecipitation and on-bead quantitative PCR analysis: a versatile and rapid ChIP procedure |pdf=|usr=}}{{25539918 }} gab.com Text Immediate chromatin immunoprecipitation and on-bead quantitative PCR analysis: a versatile and rapid ChIP procedure JO: Nucleic Acids Res http://www.kidney.de/pget.php?&tw=1&pmid=25539918 #FOAvip Genome-wide chromatin immunoprecipitation (ChIP) studies have brought significant insight into the genomic localization of chromatin-associated proteins and histone modifications. The large amount of data generated by these analyses, however, require approaches that enable rapid validation and analysis of biological relevance. Furthermore, there are still protein and modification targets that are difficult to detect using standard ChIP methods. To address these issues, we developed an immediate chromatin immunoprecipitation procedure which we call ZipChip. ZipChip significantly reduces the time and increases sensitivity allowing for rapid screening of multiple loci. Here we describe how ZipChIP enables detection of histone modifications (H3K4 mono- and trimethylation) and two yeast histone demethylases, Jhd2 and Rph1, which were previously difficult to detect using standard methods. Furthermore, we demonstrate the versatility of ZipChIP by analyzing the enrichment of the histone deacetylase Sir2 at heterochromatin in yeast and enrichment of the chromatin remodeler, PICKLE, at euchromatin in Arabidopsis thaliana. Twit Text FOAVip Immediate chromatin immunoprecipitation and on-bead quantitative PCR analysis: a versatile and rapid ChIP procedure ????Nucleic Acids Res http://www.kidney.de/pget.php?&tw=1&pmid=25539918 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=25539918 #FOAvip English Wikipedia <ref>{{Cite pmid|25539918 }}</ref> Immediate chromatin immunoprecipitation and on-bead quantitative PCR analysis: a versatile and rapid ChIP procedure #MMPMID25539918 Harmeyer KM ; South PF ; Bishop B ; Ogas J ; Briggs SD Nucleic Acids Res 2015[Mar]; 43 (6 ): e38 PMID25539918 show ga Genome-wide chromatin immunoprecipitation (ChIP) studies have brought significant insight into the genomic localization of chromatin-associated proteins and histone modifications. The large amount of data generated by these analyses, however, require approaches that enable rapid validation and analysis of biological relevance. Furthermore, there are still protein and modification targets that are difficult to detect using standard ChIP methods. To address these issues, we developed an immediate chromatin immunoprecipitation procedure which we call ZipChip. ZipChip significantly reduces the time and increases sensitivity allowing for rapid screening of multiple loci. Here we describe how ZipChIP enables detection of histone modifications (H3K4 mono- and trimethylation) and two yeast histone demethylases, Jhd2 and Rph1, which were previously difficult to detect using standard methods. Furthermore, we demonstrate the versatility of ZipChIP by analyzing the enrichment of the histone deacetylase Sir2 at heterochromatin in yeast and enrichment of the chromatin remodeler, PICKLE, at euchromatin in Arabidopsis thaliana. |Actins/genetics/metabolism [MESH] |Arabidopsis Proteins/genetics/metabolism [MESH] |Arabidopsis/genetics/metabolism [MESH] |Chromatin Immunoprecipitation/*methods/statistics & numerical data [MESH] |Chromatin/genetics/metabolism [MESH] |DNA Helicases/genetics/metabolism [MESH] |DNA-Binding Proteins/genetics/metabolism [MESH] |Genes, Fungal [MESH] |Genes, Plant [MESH] |Histone Demethylases/genetics/metabolism [MESH] |Histones/genetics/metabolism [MESH] |Jumonji Domain-Containing Histone Demethylases/genetics/metabolism [MESH] |Open Reading Frames [MESH] |Promoter Regions, Genetic [MESH] |Real-Time Polymerase Chain Reaction/*methods [MESH] |Repressor Proteins/genetics/metabolism [MESH] |Saccharomyces cerevisiae Proteins/genetics/metabolism [MESH] |Saccharomyces cerevisiae/genetics/metabolism [MESH] |Silent Information Regulator Proteins, Saccharomyces cerevisiae/genetics/metabolism [MESH]Immediate chromatin immunoprecipitation and on-bead quantitative PCR a(epmc).pdf DeepDyve Pubget Overpricing