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10.1186/s12885-015-1166-4 http://scihub22266oqcxt.onion/10.1186/s12885-015-1166-4 C4380258!4380258!25886575     free     free     free Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       BMC+Cancer 2015 ; 15 (ä): ä Nephropedia Template TP {{tp|p=25886575|t=2015. Induction of tumor initiation is dependent on CD44s in c-Met+ hepatocellular carcinoma |pdf=|usr=}}{{25886575}} gab.com Text Induction of tumor initiation is dependent on CD44s in c-Met+ hepatocellular carcinoma JO: BMC Cancer http://www.kidney.de/pget.php?&tw=1&pmid=25886575 #FOAvip Background: Hepatocellular carcinoma (HCC) patients with active hepatocyte growth factor (HGF)/c-Met signaling have a significantly worse prognosis. c-Met, a high affinity receptor for HGF, plays a critical role in cancer growth, invasion and metastasis. c-Met and CD44 have been utilized as cell surface markers to identify mesenchymal tumor-initiating stem-like cells (TISC) in several cancers including HCC. In this work, we examine the complex relationship between c-Met and CD44s (standard form), and investigate the specific role of CD44s as a tumor initiator and stemness marker in HCC. Methods: Gene and protein expression assays were utilized to investigate the relationship between CD44s and c-Met in HCC cell lines. Tumor-sphere assays and in vivo tumor assays were performed to investigate the role of CD44+ cells as TISCs. Student?s t-test or one-way ANOVA with Tukeys post-hoc test was performed to test for differences amongst groups with a p?<?.05 as significant. Results: In an immunohistochemical and immunoblot analysis of human HCC samples, we observed that more than 39% of human HCC samples express c-Met and CD44. To study the relationship between c-Met and CD44, we used MHCC97-H cells, which are CD44+/c-Met+. The knockdown of c-Met in MHCC97-H cells decreased CD44s, reduced TISC characteristics and decreased tumorsphere formation. Furthermore, we demonstrate that the inhibition of PI3K/AKT signaling decreased CD44s expression and subsequently decreased tumorsphere formation. The down-regulation of CD44s leads to a significant loss of a TISC and mesenchymal phenotype. Finally, the down-regulation of CD44s in MHCC97-H cells decreased tumor initiation in vivo compared with the scrambled control. Conclusions: In summary, our data suggest that CD44s is modulated by the c-Met-PI3K-AKT signaling cascade to support a mesenchymal and TISC phenotype in HCC cells. Moreover, c-Met could be a potential therapeutic drug for targeting HCC cells with TISC and mesenchymal phenotypes. Electronic supplementary material: The online version of this article (doi:10.1186/s12885-015-1166-4) contains supplementary material, which is available to authorized users. Twit Text FOAVip Induction of tumor initiation is dependent on CD44s in c-Met+ hepatocellular carcinoma ????BMC Cancer http://www.kidney.de/pget.php?&tw=1&pmid=25886575 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=25886575 #FOAvip English Wikipedia <ref>{{Cite pmid|25886575}}</ref> Induction of tumor initiation is dependent on CD44s in c-Met+ hepatocellular carcinoma #MMPMID25886575 Dang H; Steinway SN; Ding W; Rountree CB BMC Cancer 2015[]; 15 (ä): ä PMID25886575show ga Background: Hepatocellular carcinoma (HCC) patients with active hepatocyte growth factor (HGF)/c-Met signaling have a significantly worse prognosis. c-Met, a high affinity receptor for HGF, plays a critical role in cancer growth, invasion and metastasis. c-Met and CD44 have been utilized as cell surface markers to identify mesenchymal tumor-initiating stem-like cells (TISC) in several cancers including HCC. In this work, we examine the complex relationship between c-Met and CD44s (standard form), and investigate the specific role of CD44s as a tumor initiator and stemness marker in HCC. Methods: Gene and protein expression assays were utilized to investigate the relationship between CD44s and c-Met in HCC cell lines. Tumor-sphere assays and in vivo tumor assays were performed to investigate the role of CD44+ cells as TISCs. Student?s t-test or one-way ANOVA with Tukeys post-hoc test was performed to test for differences amongst groups with a p? äInduction of tumor initiation is dependent on CD44s in c-Met+ hepatoce(epmc).pdf DeepDyve Pubget Overpricing