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2013 ; 29
(2
): 49-53
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Biochemical and histopathological changes of intra-abdominal hypertension on the
kidneys: Experimental study in rats
#MMPMID25931845
Kö?üm A
; Borazan E
; Maralcan G
; Aytekin A
Ulus Cerrahi Derg
2013[]; 29
(2
): 49-53
PMID25931845
show ga
OBJECTIVE: This study aimed to evaluate the effects of experimentally induced
intra-abdominal hypertension on renal functions, with the combination of
biochemical and histopathological properties. MATERIAL AND METHODS: Thirty male
Wistar albino rats were used in this experimental study. Rats were divided into
four groups. Group 1 (control group, n=6) only received anesthesia. After the
induction of anesthesia, a 20 G catheter was introduced intraperitoneally to
Group 2 (sham group, n=8), Group 3 (n=8) and Group 4 (n=8). The intra-abdominal
pressure was not increased in Group 2. We applied 20 mmHg intra-peritoneal
pressure to Group 3 and 30 mmHg to Group 4 for 3 hours. After withdrawing 3 mL
intracardiac blood from all groups, the kidneys were removed for
histopathological examination. Serum urea and creatinine levels were measured in
all groups. RESULTS: Biochemical examination showed that blood urea and
creatinine levels were statistically different among all groups (p<0.05). Serum
creatinine levels in Group 3 and serum urea and creatinine levels in Group 4 were
significantly increased. In the histopathological examination, the kidneys in
Group 1 and Group 2 were classified as normal. In Group 3, areas with congestion
were detected in the glomeruli and interstitial regions. In addition to these
findings seen in Group 3, dilatation of the pelvi-caliceal structures and
proximal ureters were noticed in Group 4. CONCLUSION: The levels of serum urea
and creatinine are elevated when intra-abdominal pressure is increased due to
kidney damage. Foci of hemorrhage in the interstitial area, dilatations in the
proximal ureter, renal pelvis, and lymphatics were the pathologic findings seen
in the kidneys under such circumstances.