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10.1242/jcs.164467 http://scihub22266oqcxt.onion/10.1242/jcs.164467 C4379729!4379729!25681501     free     free     free Deprecated: Implicit conversion from float 213.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 213.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 213.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       J+Cell+Sci 2015 ; 128 (7): 1408-21 Nephropedia Template TP {{tp|p=25681501|t=2015. Sulf1 has ligand-dependent effects on canonical and non-canonical Wnt signalling |pdf=|usr=}}{{25681501}} gab.com Text Sulf1 has ligand-dependent effects on canonical and non-canonical Wnt signalling JO: J Cell Sci http://www.kidney.de/pget.php?&tw=1&pmid=25681501 #FOAvip Wnt signalling plays essential roles during embryonic development and is known to be mis-regulated in human disease. There are many molecular mechanisms that ensure tight regulation of Wnt activity. One such regulator is the heparan-sulfate-specific 6-O-endosulfatase Sulf1. Sulf1 acts extracellularly to modify the structure of heparan sulfate chains to affect the bio-availability of Wnt ligands. Sulf1 could, therefore, influence the formation of Wnt signalling complexes to modulate the activation of both canonical and non-canonical pathways. In this study, we use well-established assays in Xenopus to investigate the ability of Sulf1 to modify canonical and non-canonical Wnt signalling. In addition, we model the ability of Sulf1 to influence morphogen gradients using fluorescently tagged Wnt ligands in ectodermal explants. We show that Sulf1 overexpression has ligand-specific effects on Wnt signalling: it affects membrane accumulation and extracellular levels of tagged Wnt8a and Wnt11b ligands differently, and inhibits the activity of canonical Wnt8a but enhances the activity of non-canonical Wnt11b. Twit Text FOAVip Sulf1 has ligand-dependent effects on canonical and non-canonical Wnt signalling ????J Cell Sci http://www.kidney.de/pget.php?&tw=1&pmid=25681501 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=25681501 #FOAvip English Wikipedia <ref>{{Cite pmid|25681501}}</ref> Sulf1 has ligand-dependent effects on canonical and non-canonical Wnt signalling #MMPMID25681501 Fellgett SW; Maguire RJ; Pownall ME J Cell Sci 2015[Apr]; 128 (7): 1408-21 PMID25681501show ga Wnt signalling plays essential roles during embryonic development and is known to be mis-regulated in human disease. There are many molecular mechanisms that ensure tight regulation of Wnt activity. One such regulator is the heparan-sulfate-specific 6-O-endosulfatase Sulf1. Sulf1 acts extracellularly to modify the structure of heparan sulfate chains to affect the bio-availability of Wnt ligands. Sulf1 could, therefore, influence the formation of Wnt signalling complexes to modulate the activation of both canonical and non-canonical pathways. In this study, we use well-established assays in Xenopus to investigate the ability of Sulf1 to modify canonical and non-canonical Wnt signalling. In addition, we model the ability of Sulf1 to influence morphogen gradients using fluorescently tagged Wnt ligands in ectodermal explants. We show that Sulf1 overexpression has ligand-specific effects on Wnt signalling: it affects membrane accumulation and extracellular levels of tagged Wnt8a and Wnt11b ligands differently, and inhibits the activity of canonical Wnt8a but enhances the activity of non-canonical Wnt11b. äSulf1 has ligand-dependent effects on canonical and non-canonical Wnt (epmc).pdf DeepDyve Pubget Overpricing