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Huaier Cream Protects against Adriamycin-Induced Nephropathy by Restoring
Mitochondrial Function via PGC-1? Upregulation
#MMPMID25861251
Che R
; Zhu C
; Ding G
; Zhao M
; Bai M
; Jia Z
; Zhang A
; Huang S
PPAR Res
2015[]; 2015
(?): 720383
PMID25861251
show ga
The mechanism by which Huaier, a Chinese traditional medicine, protects podocytes
remains unclear. We designed the present study to examine whether mitochondrial
function restored by PGC-1? serves as the major target of Huaier cream in
protecting ADR nephropathy. After ADR administration, the podocytes exhibited
remarkable cell injury and mitochondrial dysfunction. Additionally, ADR also
reduced PGC-1? both in vivo and in vitro. Following the Huaier treatment, the
notable downregulation of PGC-1? and its downstream molecule mitochondrial
transcription factor A (TFAM) were almost entirely blocked. Correspondingly,
Huaier markedly ameliorated ADR-induced podocyte injury and mitochondrial
dysfunction in both rat kidneys and incubated cells as it inhibited the decrease
of nephrin and podocin expression, mtDNA copy number, MMP, and ATP content.
Transmission electron microscopy result also showed that Huaier protected
mitochondria against ADR-induced severe mitophagy and abnormal changes of
ultrastructural morphology. In conclusion, Huaier can protect podocytes against
ADR-induced cytotoxicity possibly by reversing the dysfunction of mitochondria
via PGC-1? overexpression, which may be a novel therapeutic drug target in
glomerular diseases.
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