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10.1063/1.4915612 http://scihub22266oqcxt.onion/10.1063/1.4915612 C4376752!4376752!25825615     free     free     free Deprecated: Implicit conversion from float 217.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 217.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 217.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Biomicrofluidics 2015 ; 9 (2): ä Nephropedia Template TP {{tp|p=25825615|t=2015. Embryo formation from low sperm concentration by using dielectrophoretic force |pdf=|usr=}}{{25825615}} gab.com Text Embryo formation from low sperm concentration by using dielectrophoretic force JO: Biomicrofluidics http://www.kidney.de/pget.php?&tw=1&pmid=25825615 #FOAvip A biochip system imitates the oviduct of mammals with a microfluidic channel to achieve fertilization in vitro of imprinting-control-region (ICR) mice. We apply a method to manipulate and to position the oocyte and the sperm of ICR mice at the same time in our microfluidic channel with a positive dielectrophoretic (DEP) force. The positive dielectrophoretic response of the oocyte and sperm was exhibited under applied bias conditions AC 10 Vpp waveform, 1?MHz, 10?min. With this method, the concentration of sperm in the vicinity of the oocyte was increased and enhanced the probability of natural fertilization. We used commercial numerical software (CFDRC-ACE+) to simulate the square of the electric field and analyzed the location at which the oocyte and sperm are trapped. The microfluidic devices were designed and fabricated with poly(dimethylsiloxane). The results of our experiments indicate that a positive DEP served to drive the position of the oocyte and the sperm to natural fertilization (average rate of fertilization 51.58%) in our microchannel structures at insemination concentration 1.5?×?106 sperm ml?1. Embryos were cultured to two cells after 24?h and four cells after 48?h. Twit Text FOAVip Embryo formation from low sperm concentration by using dielectrophoretic force ????Biomicrofluidics http://www.kidney.de/pget.php?&tw=1&pmid=25825615 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=25825615 #FOAvip English Wikipedia <ref>{{Cite pmid|25825615}}</ref> Embryo formation from low sperm concentration by using dielectrophoretic force #MMPMID25825615 Huang HY; Huang YH; Kao WL; Yao DJ Biomicrofluidics 2015[Mar]; 9 (2): ä PMID25825615show ga A biochip system imitates the oviduct of mammals with a microfluidic channel to achieve fertilization in vitro of imprinting-control-region (ICR) mice. We apply a method to manipulate and to position the oocyte and the sperm of ICR mice at the same time in our microfluidic channel with a positive dielectrophoretic (DEP) force. The positive dielectrophoretic response of the oocyte and sperm was exhibited under applied bias conditions AC 10 Vpp waveform, 1?MHz, 10?min. With this method, the concentration of sperm in the vicinity of the oocyte was increased and enhanced the probability of natural fertilization. We used commercial numerical software (CFDRC-ACE+) to simulate the square of the electric field and analyzed the location at which the oocyte and sperm are trapped. The microfluidic devices were designed and fabricated with poly(dimethylsiloxane). The results of our experiments indicate that a positive DEP served to drive the position of the oocyte and the sperm to natural fertilization (average rate of fertilization 51.58%) in our microchannel structures at insemination concentration 1.5?×?106 sperm ml?1. Embryos were cultured to two cells after 24?h and four cells after 48?h. äEmbryo formation from low sperm concentration by using dielectrophoret(epmc).pdf DeepDyve Pubget Overpricing