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10.1016/j.celrep.2015.02.040 http://scihub22266oqcxt.onion/10.1016/j.celrep.2015.02.040 C4376630!4376630!25772367     free     free     free Deprecated: Implicit conversion from float 227.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 227.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 227.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 227.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 227.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Cell+Rep 2015 ; 10 (11): 1828-35 Nephropedia Template TP {{tp|p=25772367|t=2015. Targeted Germline Modifications in Rats using CRISPR/Cas9 and Spermatogonial Stem Cells |pdf=|usr=}}{{25772367}} gab.com Text Targeted Germline Modifications in Rats using CRISPR/Cas9 and Spermatogonial Stem Cells JO: Cell Rep http://www.kidney.de/pget.php?&tw=1&pmid=25772367 #FOAvip Organisms with targeted genomic modifications are efficiently produced by gene editing in embryos using CRISPR/Cas9 RNA-guided DNA endonuclease. Here, to facilitate germline editing in rats, we used CRISPR/Cas9 to catalyze targeted genomic mutations in rat spermatogonial stem cell cultures. CRISPR/Cas9-modified spermatogonia regenerated spermatogenesis and displayed long-term sperm forming potential following transplantation into rat testes. Targeted germline mutations in Epsti1 and Erbb3 were vertically transmitted from recipients to exclusively generate ?pure?, non-mosaic mutant progeny. Epsti1 mutant rats were produced with or without genetically selecting donor spermatogonia. Monoclonal enrichment of Erbb3-null germlines unmasked recessive spermatogenesis defects in culture that were buffered in recipients, yielding mutant progeny isogenic at targeted alleles. Thus, spermatogonial gene editing with CRISPR/Cas9 provided a platform to generate targeted germline mutations in rats, and to study spermatogenesis. Twit Text FOAVip Targeted Germline Modifications in Rats using CRISPR/Cas9 and Spermatogonial Stem Cells ????Cell Rep http://www.kidney.de/pget.php?&tw=1&pmid=25772367 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=25772367 #FOAvip English Wikipedia <ref>{{Cite pmid|25772367}}</ref> Targeted Germline Modifications in Rats using CRISPR/Cas9 and Spermatogonial Stem Cells #MMPMID25772367 Chapman KM; Medrano GA; Jaichander P; Chaudhary J; Waits AE; Nobrega MA; Hotaling JM; Ober C; Hamra FK Cell Rep 2015[Mar]; 10 (11): 1828-35 PMID25772367show ga Organisms with targeted genomic modifications are efficiently produced by gene editing in embryos using CRISPR/Cas9 RNA-guided DNA endonuclease. Here, to facilitate germline editing in rats, we used CRISPR/Cas9 to catalyze targeted genomic mutations in rat spermatogonial stem cell cultures. CRISPR/Cas9-modified spermatogonia regenerated spermatogenesis and displayed long-term sperm forming potential following transplantation into rat testes. Targeted germline mutations in Epsti1 and Erbb3 were vertically transmitted from recipients to exclusively generate ?pure?, non-mosaic mutant progeny. Epsti1 mutant rats were produced with or without genetically selecting donor spermatogonia. Monoclonal enrichment of Erbb3-null germlines unmasked recessive spermatogenesis defects in culture that were buffered in recipients, yielding mutant progeny isogenic at targeted alleles. Thus, spermatogonial gene editing with CRISPR/Cas9 provided a platform to generate targeted germline mutations in rats, and to study spermatogenesis. äTargeted Germline Modifications in Rats using CRISPR/Cas9 and Spermato(epmc).pdf DeepDyve Pubget Overpricing