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10.1038/nature14263 http://scihub22266oqcxt.onion/10.1038/nature14263 C4376618!4376618!25799993     free     free     free Deprecated: Implicit conversion from float 225.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Nature 2015 ; 519 (7544): 486-90 Nephropedia Template TP {{tp|p=25799993|t=2015. Structural imprints in vivo decode RNA regulatory mechanisms |pdf=|usr=}}{{25799993}} gab.com Text Structural imprints in vivo decode RNA regulatory mechanisms JO: Nature http://www.kidney.de/pget.php?&tw=1&pmid=25799993 #FOAvip Visualizing the physical basis for molecular behavior inside living cells is a grand challenge in biology. RNAs are central to biological regulation, and RNA?s ability to adopt specific structures intimately controls every step of the gene expression program1. However, our understanding of physiological RNA structures is limited; current in vivo RNA structure profiles view only two of four nucleotides that make up RNA2,3. Here we present a novel biochemical approach, In Vivo Click SHAPE (icSHAPE), that enables the first global view of RNA secondary structures of all four bases in living cells. icSHAPE of mouse embryonic stem cell transcriptome versus purified RNA folded in vitro shows that the structural dynamics of RNA in the cellular environment distinguishes different classes of RNAs and regulatory elements. Structural signatures at translational start sites and ribosome pause sites are conserved from in vitro, suggesting that these RNA elements are programmed by sequence. In contrast, focal structural rearrangements in vivo reveal precise interfaces of RNA with RNA binding proteins or RNA modification sites that are consistent with atomic-resolution structural data. Such dynamic structural footprints enable accurate prediction of RNA-protein interactions and N6-methyladenosine (m6A) modification genome-wide. These results open the door for structural genomics of RNA in living cells and reveal key physiological structures controlling gene expression. Twit Text FOAVip Structural imprints in vivo decode RNA regulatory mechanisms ????Nature http://www.kidney.de/pget.php?&tw=1&pmid=25799993 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=25799993 #FOAvip English Wikipedia <ref>{{Cite pmid|25799993}}</ref> Structural imprints in vivo decode RNA regulatory mechanisms #MMPMID25799993 Spitale RC; Flynn RA; Zhang QC; Crisalli P; Lee B; Jung JW; Kuchelmeister HY; Batista PJ; Torre EA; Kool ET; Chang HY Nature 2015[Mar]; 519 (7544): 486-90 PMID25799993show ga Visualizing the physical basis for molecular behavior inside living cells is a grand challenge in biology. RNAs are central to biological regulation, and RNA?s ability to adopt specific structures intimately controls every step of the gene expression program1. However, our understanding of physiological RNA structures is limited; current in vivo RNA structure profiles view only two of four nucleotides that make up RNA2,3. Here we present a novel biochemical approach, In Vivo Click SHAPE (icSHAPE), that enables the first global view of RNA secondary structures of all four bases in living cells. icSHAPE of mouse embryonic stem cell transcriptome versus purified RNA folded in vitro shows that the structural dynamics of RNA in the cellular environment distinguishes different classes of RNAs and regulatory elements. Structural signatures at translational start sites and ribosome pause sites are conserved from in vitro, suggesting that these RNA elements are programmed by sequence. In contrast, focal structural rearrangements in vivo reveal precise interfaces of RNA with RNA binding proteins or RNA modification sites that are consistent with atomic-resolution structural data. Such dynamic structural footprints enable accurate prediction of RNA-protein interactions and N6-methyladenosine (m6A) modification genome-wide. These results open the door for structural genomics of RNA in living cells and reveal key physiological structures controlling gene expression. äStructural imprints in vivo decode RNA regulatory mechanisms (epmc).pdf DeepDyve Pubget Overpricing