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10.1016/j.bpj.2014.12.050 http://scihub22266oqcxt.onion/10.1016/j.bpj.2014.12.050 C4375424!4375424!25762322     free     free     free Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Biophys+J 2015 ; 108 (5): 1104-13 Nephropedia Template TP {{tp|p=25762322|t=2015. Cytoskeletal Pinning Controls Phase Separation in Multicomponent Lipid Membranes |pdf=|usr=}}{{25762322}} gab.com Text Cytoskeletal Pinning Controls Phase Separation in Multicomponent Lipid Membranes JO: Biophys J http://www.kidney.de/pget.php?&tw=1&pmid=25762322 #FOAvip We study the effect of a minimal cytoskeletal network formed on the surface of giant unilamellar vesicles by the prokaryotic tubulin homolog, FtsZ, on phase separation in freestanding lipid membranes. FtsZ has been modified to interact with the membrane through a membrane targeting sequence from the prokaryotic protein MinD. FtsZ with the attached membrane targeting sequence efficiently forms a highly interconnected network on membranes with a concentration-dependent mesh size, much similar to the eukaryotic cytoskeletal network underlying the plasma membrane. Using giant unilamellar vesicles formed from a quaternary lipid mixture, we demonstrate that the artificial membrane-associated cytoskeleton, on the one hand, suppresses large-scale phase separation below the phase transition temperature, and, on the other hand, preserves phase separation above the transition temperature. Our experimental observations support the ideas put forward in our previous simulation study: In particular, the picket fence effect on phase separation may explain why micrometer-scale membrane domains are observed in isolated, cytoskeleton-free giant plasma membrane vesicles, but not in intact cell membranes. The experimentally observed suppression of large-scale phase separation much below the transition temperatures also serves as an argument in favor of the cryoprotective role of the cytoskeleton. Twit Text FOAVip Cytoskeletal Pinning Controls Phase Separation in Multicomponent Lipid Membranes ????Biophys J http://www.kidney.de/pget.php?&tw=1&pmid=25762322 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=25762322 #FOAvip English Wikipedia <ref>{{Cite pmid|25762322}}</ref> Cytoskeletal Pinning Controls Phase Separation in Multicomponent Lipid Membranes #MMPMID25762322 Arumugam S; Petrov E; Schwille P Biophys J 2015[Mar]; 108 (5): 1104-13 PMID25762322show ga We study the effect of a minimal cytoskeletal network formed on the surface of giant unilamellar vesicles by the prokaryotic tubulin homolog, FtsZ, on phase separation in freestanding lipid membranes. FtsZ has been modified to interact with the membrane through a membrane targeting sequence from the prokaryotic protein MinD. FtsZ with the attached membrane targeting sequence efficiently forms a highly interconnected network on membranes with a concentration-dependent mesh size, much similar to the eukaryotic cytoskeletal network underlying the plasma membrane. Using giant unilamellar vesicles formed from a quaternary lipid mixture, we demonstrate that the artificial membrane-associated cytoskeleton, on the one hand, suppresses large-scale phase separation below the phase transition temperature, and, on the other hand, preserves phase separation above the transition temperature. Our experimental observations support the ideas put forward in our previous simulation study: In particular, the picket fence effect on phase separation may explain why micrometer-scale membrane domains are observed in isolated, cytoskeleton-free giant plasma membrane vesicles, but not in intact cell membranes. The experimentally observed suppression of large-scale phase separation much below the transition temperatures also serves as an argument in favor of the cryoprotective role of the cytoskeleton. äCytoskeletal Pinning Controls Phase Separation in Multicomponent Lipid(epmc).pdf DeepDyve Pubget Overpricing