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Telocytes accompanying cardiomyocyte in primary culture: two- and
three-dimensional culture environment
#MMPMID21158014
Zhou J
; Zhang Y
; Wen X
; Cao J
; Li D
; Lin Q
; Wang H
; Liu Z
; Duan C
; Wu K
; Wang C
J Cell Mol Med
2010[Nov]; 14
(11
): 2641-5
PMID21158014
show ga
Recently, the presence of telocytes was demonstrated in human and mammalian
tissues and organs (digestive and extra-digestive organs, genitourinary organs,
heart, placenta, lungs, pleura, striated muscle). Noteworthy, telocytes seem to
play a significant role in the normal function and regeneration of myocardium. By
cultures of telocytes in two- and three-dimensional environment we aimed to study
the typical morphological features as well as functionality of telocytes, which
will provide important support to understand their in vivo roles. Neonatal rat
cardiomyocytes were isolated and cultured as seeding cells in vitro in
two-dimensional environment. Furthermore, engineered myocardium tissue was
constructed from isolated cells in three-dimensional collagen/Matrigel scaffolds.
The identification of telocytes was performed by using histological and
immunohistochemical methods. The results showed that typical telocytes are
distributed among cardiomyocytes, connecting them by long telopodes. Telocytes
have a typical fusiform cell body with two or three long moniliform telopodes, as
main characteristics. The vital methylene blue staining showed the existence of
telocytes in primary culture. Immunohistochemistry demonstrated that some c-kit
or CD34 immuno-positive cells in engineered heart tissue had the morphology of
telocytes, with a typical fusiform cell body and long moniliform telopodes. Also,
a significant number of vimentin+ telocytes were present within engineered heart
tissue. We suggest that the model of three-dimensional engineered heart tissue
could be useful for the ongoing research on the functional relationships of
telocytes with cardiomyocytes. Because the heart has the necessary potential of
changing the muscle and non-muscle cells during the lifetime, telocytes might
play an active role in the heart regeneration process. Moreover, telocytes might
be a useful tool for cardiac tissue engineering.
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