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10.3389/fphys.2015.00085 http://scihub22266oqcxt.onion/10.3389/fphys.2015.00085 C4371692!4371692!25852572     free     free     free Deprecated: Implicit conversion from float 225.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 225.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 225.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 225.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 225.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 225.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 225.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 259.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Front+Physiol 2015 ; 6 (ä): ä Nephropedia Template TP {{tp|p=25852572|t=2015. AMP-activated protein kinase controls exercise training- and AICAR-induced increases in SIRT3 and MnSOD |pdf=|usr=}}{{25852572}} gab.com Text AMP-activated protein kinase controls exercise training- and AICAR-induced increases in SIRT3 and MnSOD JO: Front Physiol http://www.kidney.de/pget.php?&tw=1&pmid=25852572 #FOAvip The mitochondrial protein deacetylase sirtuin (SIRT) 3 may mediate exercise training-induced increases in mitochondrial biogenesis and improvements in reactive oxygen species (ROS) handling. We determined the requirement of AMP-activated protein kinase (AMPK) for exercise training-induced increases in skeletal muscle abundance of SIRT3 and other mitochondrial proteins. Exercise training for 6.5 weeks increased SIRT3 (p < 0.01) and superoxide dismutase 2 (MnSOD; p < 0.05) protein abundance in quadriceps muscle of wild-type (WT; n = 13?15), but not AMPK ?2 kinase dead (KD; n = 12?13) mice. We also observed a strong trend for increased MnSOD abundance in exercise-trained skeletal muscle of healthy humans (p = 0.051; n = 6). To further elucidate a role for AMPK in mediating these effects, we treated WT (n = 7?8) and AMPK ?2 KD (n = 7?9) mice with 5-amino-1-?-D-ribofuranosyl-imidazole-4-carboxamide (AICAR). Four weeks of daily AICAR injections (500 mg/kg) resulted in AMPK-dependent increases in SIRT3 (p < 0.05) and MnSOD (p < 0.01) in WT, but not AMPK ?2 KD mice. We also tested the effect of repeated AICAR treatment on mitochondrial protein levels in mice lacking the transcriptional coactivator peroxisome proliferator-activated receptor ?-coactivator 1? (PGC-1? KO; n = 9?10). Skeletal muscle SIRT3 and MnSOD protein abundance was reduced in sedentary PGC-1? KO mice (p < 0.01) and AICAR-induced increases in SIRT3 and MnSOD protein abundance was only observed in WT mice (p < 0.05). Finally, the acetylation status of SIRT3 target lysine residues on MnSOD (K122) or oligomycin-sensitivity conferring protein (OSCP; K139) was not altered in either mouse or human skeletal muscle in response to acute exercise. We propose an important role for AMPK in regulating mitochondrial function and ROS handling in skeletal muscle in response to exercise training. Twit Text FOAVip AMP-activated protein kinase controls exercise training- and AICAR-induced increases in SIRT3 and MnSOD ????Front Physiol http://www.kidney.de/pget.php?&tw=1&pmid=25852572 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=25852572 #FOAvip English Wikipedia <ref>{{Cite pmid|25852572}}</ref> AMP-activated protein kinase controls exercise training- and AICAR-induced increases in SIRT3 and MnSOD #MMPMID25852572 Brandauer J; Andersen MA; Kellezi H; Risis S; Frøsig C; Vienberg SG; Treebak JT Front Physiol 2015[]; 6 (ä): ä PMID25852572show ga The mitochondrial protein deacetylase sirtuin (SIRT) 3 may mediate exercise training-induced increases in mitochondrial biogenesis and improvements in reactive oxygen species (ROS) handling. We determined the requirement of AMP-activated protein kinase (AMPK) for exercise training-induced increases in skeletal muscle abundance of SIRT3 and other mitochondrial proteins. Exercise training for 6.5 weeks increased SIRT3 (p < 0.01) and superoxide dismutase 2 (MnSOD; p < 0.05) protein abundance in quadriceps muscle of wild-type (WT; n = 13?15), but not AMPK ?2 kinase dead (KD; n = 12?13) mice. We also observed a strong trend for increased MnSOD abundance in exercise-trained skeletal muscle of healthy humans (p = 0.051; n = 6). To further elucidate a role for AMPK in mediating these effects, we treated WT (n = 7?8) and AMPK ?2 KD (n = 7?9) mice with 5-amino-1-?-D-ribofuranosyl-imidazole-4-carboxamide (AICAR). Four weeks of daily AICAR injections (500 mg/kg) resulted in AMPK-dependent increases in SIRT3 (p < 0.05) and MnSOD (p < 0.01) in WT, but not AMPK ?2 KD mice. We also tested the effect of repeated AICAR treatment on mitochondrial protein levels in mice lacking the transcriptional coactivator peroxisome proliferator-activated receptor ?-coactivator 1? (PGC-1? KO; n = 9?10). Skeletal muscle SIRT3 and MnSOD protein abundance was reduced in sedentary PGC-1? KO mice (p < 0.01) and AICAR-induced increases in SIRT3 and MnSOD protein abundance was only observed in WT mice (p < 0.05). Finally, the acetylation status of SIRT3 target lysine residues on MnSOD (K122) or oligomycin-sensitivity conferring protein (OSCP; K139) was not altered in either mouse or human skeletal muscle in response to acute exercise. We propose an important role for AMPK in regulating mitochondrial function and ROS handling in skeletal muscle in response to exercise training. äAMP-activated protein kinase controls exercise training- and AICAR-ind(epmc).pdf DeepDyve Pubget Overpricing