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10.1371/journal.pone.0119912 http://scihub22266oqcxt.onion/10.1371/journal.pone.0119912 C4370640!4370640 !25799367     free     free     free Warning: file_get_contents(https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pubmed&id=25799367 &cmd=llinks): Failed to open stream: HTTP request failed! 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Silencing TRPM7 in mouse cortical astrocytes impairs cell proliferation and migration via ERK and JNK signaling pathways |pdf=|usr=}}{{25799367 }} gab.com Text Silencing TRPM7 in mouse cortical astrocytes impairs cell proliferation and migration via ERK and JNK signaling pathways JO: PLoS One http://www.kidney.de/pget.php?&tw=1&pmid=25799367 #FOAvip Transient receptor potential melastatin 7 (TRPM7), a non-selective cation channel, is highly expressed expressed in the brain and plays a critical role in ischemic neuronal death. Astrocyte, the most abundant cell type in central nervous system (CNS), exerts many essential functions in the physiological and pathological conditions. Here we investigated the expression and functions of the TRPM7 channel in mouse cortical astrocytes. Using reverse transcription (RT)-PCR, immunostaining, western blot and patch clamp recording, we showed that functional TRPM7 channel is expressed in cultured mouse cortical astrocytes. Knocking down TRPM7 with specific siRNA impairs the proliferation and migration of astrocytes by 40.2% ± 3.9% and 40.1% ± 11.5%, respectively. Consistently, inhibition of TRPM7 with 2-aminoethoxydiphenyl borate (2-APB) also decreases astrocyte proliferation and migration by 46.1% ± 2.5% and 64.2% ± 2.4%. MAPKs and Akt signaling pathways have been shown to be implicated in TRPM7-mediated responses including cell proliferation and migration. Our data show that suppression of TRPM7 in astrocytes reduces the phosphorylation of extracellular signal-regulated kinases (ERK) and c-Jun N-terminal kinases (JNK), but not p38 mitogen-activated protein kinase and Akt. In addition, TRPM7, as a cation channel, has been involved in the Ca²? and Mg²? homeostasis in several types of cells. In our study, we found that silencing TRPM7 decreases the intracellular basal Mg²? concentration without affecting Ca²? concentration in astrocytes. However, an addition of Mg²? to the growth medium could not rescue the impaired proliferation of astrocytes. Together, our data suggest that TRPM7 channel may play a critical role in the proliferation and migration of astrocytes via the ERK and JNK pathways. Twit Text FOAVip Silencing TRPM7 in mouse cortical astrocytes impairs cell proliferation and migration via ERK and JNK signaling pathways ????PLoS One http://www.kidney.de/pget.php?&tw=1&pmid=25799367 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=25799367 #FOAvip English Wikipedia <ref>{{Cite pmid|25799367 }}</ref> Silencing TRPM7 in mouse cortical astrocytes impairs cell proliferation and migration via ERK and JNK signaling pathways #MMPMID25799367 Zeng Z ; Leng T ; Feng X ; Sun H ; Inoue K ; Zhu L ; Xiong ZG PLoS One 2015[]; 10 (3 ): e0119912 PMID25799367 show ga Transient receptor potential melastatin 7 (TRPM7), a non-selective cation channel, is highly expressed expressed in the brain and plays a critical role in ischemic neuronal death. Astrocyte, the most abundant cell type in central nervous system (CNS), exerts many essential functions in the physiological and pathological conditions. Here we investigated the expression and functions of the TRPM7 channel in mouse cortical astrocytes. Using reverse transcription (RT)-PCR, immunostaining, western blot and patch clamp recording, we showed that functional TRPM7 channel is expressed in cultured mouse cortical astrocytes. Knocking down TRPM7 with specific siRNA impairs the proliferation and migration of astrocytes by 40.2% ± 3.9% and 40.1% ± 11.5%, respectively. Consistently, inhibition of TRPM7 with 2-aminoethoxydiphenyl borate (2-APB) also decreases astrocyte proliferation and migration by 46.1% ± 2.5% and 64.2% ± 2.4%. MAPKs and Akt signaling pathways have been shown to be implicated in TRPM7-mediated responses including cell proliferation and migration. Our data show that suppression of TRPM7 in astrocytes reduces the phosphorylation of extracellular signal-regulated kinases (ERK) and c-Jun N-terminal kinases (JNK), but not p38 mitogen-activated protein kinase and Akt. In addition, TRPM7, as a cation channel, has been involved in the Ca²? and Mg²? homeostasis in several types of cells. In our study, we found that silencing TRPM7 decreases the intracellular basal Mg²? concentration without affecting Ca²? concentration in astrocytes. However, an addition of Mg²? to the growth medium could not rescue the impaired proliferation of astrocytes. Together, our data suggest that TRPM7 channel may play a critical role in the proliferation and migration of astrocytes via the ERK and JNK pathways. |*Cell Movement [MESH] |*Cell Proliferation [MESH] |Animals [MESH] |Apoptosis [MESH] |Astrocytes/*cytology/metabolism [MESH] |Blotting, Western [MESH] |Calcium/metabolism [MESH] |Cells, Cultured [MESH] |Cerebral Cortex/*cytology/metabolism [MESH] |Extracellular Signal-Regulated MAP Kinases/genetics/*metabolism [MESH] |Gene Silencing [MESH] |HEK293 Cells [MESH] |Humans [MESH] |Immunoenzyme Techniques [MESH] |JNK Mitogen-Activated Protein Kinases/genetics/*metabolism [MESH] |Mice [MESH] |Phosphorylation [MESH] |Protein Serine-Threonine Kinases/*antagonists & inhibitors/genetics/metabolism [MESH] |RNA, Messenger/genetics [MESH] |RNA, Small Interfering/genetics [MESH] |Real-Time Polymerase Chain Reaction [MESH] |Reverse Transcriptase Polymerase Chain Reaction [MESH] |Signal Transduction [MESH]Silencing TRPM7 in mouse cortical astrocytes impairs cell proliferatio(epmc).pdf DeepDyve Pubget Overpricing